Middelbos Ingmar S, Vester Brittany M, Karr-Lilienthal Lisa K, Schook Lawrence B, Swanson Kelly S
Department of Animal Sciences, University of Illinois, Urbana, Illinois, United States of America.
PLoS One. 2009;4(2):e4481. doi: 10.1371/journal.pone.0004481. Epub 2009 Feb 16.
We evaluated gene transcription in canine skeletal muscle (biceps femoris) using microarray analysis to identify effects of age and diet on gene expression. Twelve female beagles were used (six 1-year olds and six 12-year olds) and they were fed one of two experimental diets for 12 months. One diet contained primarily plant-based protein sources (PPB), whereas the second diet contained primarily animal-based protein sources (APB). Affymetrix GeneChip Canine Genome Arrays were used to hybridize extracted RNA. Age had the greatest effect on gene transcription (262 differentially expressed genes), whereas the effect of diet was relatively small (22 differentially expressed genes). Effects of age (regardless of diet) were most notable on genes related to metabolism, cell cycle and cell development, and transcription function. All these genes were predominantly down-regulated in geriatric dogs. Age-affected genes that were differentially expressed on only one of two diets were primarily noted in the PPB diet group (144/165 genes). Again, genes related to cell cycle (22/35) and metabolism (15/19) had predominantly decreased transcription in geriatric dogs, but 6/8 genes related to muscle development had increased expression. Effects of diet on muscle gene expression were mostly noted in geriatric dogs, but no consistent patterns in transcription were observed. The insight these data provide into gene expression profiles of canine skeletal muscle as affected by age, could serve as a foundation for future research pertaining to age-related muscle diseases.
我们使用微阵列分析评估了犬骨骼肌（股二头肌）中的基因转录，以确定年龄和饮食对基因表达的影响。使用了12只雌性比格犬（6只1岁和6只12岁），并给它们喂食两种实验性饮食之一，持续12个月。一种饮食主要包含植物性蛋白质来源（PPB），而第二种饮食主要包含动物性蛋白质来源（APB）。使用Affymetrix GeneChip犬基因组阵列对提取的RNA进行杂交。年龄对基因转录的影响最大（262个差异表达基因），而饮食的影响相对较小（22个差异表达基因）。年龄（无论饮食如何）对与代谢、细胞周期和细胞发育以及转录功能相关的基因影响最为显著。所有这些基因在老年犬中主要下调。仅在两种饮食之一上差异表达的受年龄影响的基因主要在PPB饮食组中被发现（144/165个基因）。同样，与细胞周期（22/35）和代谢（15/19）相关的基因在老年犬中转录主要减少，但与肌肉发育相关的6/8个基因表达增加。饮食对肌肉基因表达的影响主要在老年犬中被发现，但未观察到一致的转录模式。这些数据为受年龄影响的犬骨骼肌基因表达谱提供的见解，可为未来与年龄相关的肌肉疾病研究奠定基础。
