通过毛细管柱气液色谱法对不同念珠菌属进行脂肪酸分析。

Fatty acid analysis of different Candida species by capillary column gas-liquid chromatography.

作者信息

Mayatepek E, Herz A, Leichsenring M, Kappe R

机构信息

Institute of Hygiene, University of Heidelberg, Germany.

出版信息

Mycoses. 1991 Jan-Feb;34(1-2):53-7. doi: 10.1111/j.1439-0507.1991.tb00619.x.

DOI:10.1111/j.1439-0507.1991.tb00619.x

PMID:1922189

Abstract

Gas-liquid chromatography was used to analyse the fatty acid composition of whole-cell hydrolysates of 40 yeast strains representing 5 Candida species and Torulopsis glabrata, T. glabrata could be easily distinguished from all other Candida spp. by the absence of C18:3. Candida albicans, C. krusei, C. parapsilosis, C. pseudotropicalis and C. tropicalis showed 10 major peaks in characteristic proportions. C. parapsilosis showed a high C16:0/C16:1 ratio (greater than 4.5), whereas C. pseudotropicalis was characterized by a C18:1/C16:0 ratio of less than 2.0. A high C18:3 concentration (greater than 10%) was typical for C. krusei (C18:2/C18:3 ratio less than or equal to 1.0). Our data reveal characteristic patterns of cellular fatty acid composition of T. glabrata, C. krusei, C. parapsilosis and C. pseudotropicalis which can be used for laboratory identification.

摘要

采用气-液色谱法分析了代表5种念珠菌属和光滑球拟酵母的40株酵母菌株全细胞水解产物的脂肪酸组成。光滑球拟酵母因缺乏C18:3，可轻易与所有其他念珠菌属区分开来。白色念珠菌、克鲁斯念珠菌、近平滑念珠菌、伪热带念珠菌和热带念珠菌呈现出特征比例的10个主要峰。近平滑念珠菌的C16:0/C16:1比值较高（大于4.5），而伪热带念珠菌的特征是C18:1/C16:0比值小于2.0。克鲁斯念珠菌的典型特征是C18:3浓度较高（大于10%）（C18:2/C18:3比值小于或等于1.0）。我们的数据揭示了光滑球拟酵母、克鲁斯念珠菌、近平滑念珠菌和伪热带念珠菌细胞脂肪酸组成的特征模式，可用于实验室鉴定。