Taweechotipatr Malai, Iyer Chandra, Spinler Jennifer K, Versalovic James, Tumwasorn Somying
Inter-Department of Medical Microbiology, Graduate School, Chulalongkorn University, Bangkok, Thailand.
FEMS Microbiol Lett. 2009 Apr;293(1):65-72. doi: 10.1111/j.1574-6968.2009.01506.x. Epub 2009 Feb 12.
Human-derived lactobacilli were isolated from fecal samples of healthy volunteers. Forty-six isolates from different volunteers were selected and investigated for their immunomodulatory properties. Conditioned medium from each isolate was assessed for its effect on tumor necrosis factor (TNF) production in lipopolysaccharide-activated THP-1 monocytes. Of 46 Lactobacillus isolates, 12 significantly inhibited TNF production in varying magnitude. Lactobacillus strain TH58 displayed the most potent TNF-inhibitory activity (70% inhibition). In contrast, Lactobacillus strain TH14 exhibited immunostimulatory property by activating TNF production in THP-1 monocytes. Lactobacillus TH14 induced nuclear factor-kappaB (NF-kappaB) activation in the absence of lipopolysaccharide stimulation, whereas Lactobacillus TH58 had no effect on NF-kappaB signaling, irrespective of lipopolysaccharide stimulation. Strain TH58 was identified as Lactobacillus saerimneri and strain TH14 as Lactobacillus ruminis by sequence analysis of their 16S rRNA genes. This is the first report of a human isolate of L. saerimneri with TNF-inhibitory activity and L. ruminis, an indigenous species to humans, with TNF stimulatory activity. Our data suggest the potential use of these two strains as immunoprobiotic candidates.
从健康志愿者的粪便样本中分离出人类源乳酸杆菌。从不同志愿者中挑选出46株分离株，并对其免疫调节特性进行研究。评估每株分离株的条件培养基对脂多糖激活的THP-1单核细胞中肿瘤坏死因子（TNF）产生的影响。在46株乳酸杆菌分离株中，有12株在不同程度上显著抑制TNF的产生。乳酸杆菌菌株TH58表现出最有效的TNF抑制活性（70%抑制率）。相比之下，乳酸杆菌菌株TH14通过激活THP-1单核细胞中的TNF产生而表现出免疫刺激特性。乳酸杆菌TH14在无脂多糖刺激的情况下诱导核因子-κB（NF-κB）激活，而乳酸杆菌TH58无论有无脂多糖刺激，对NF-κB信号传导均无影响。通过对其16S rRNA基因的序列分析，将菌株TH58鉴定为萨氏乳酸杆菌，菌株TH14鉴定为瘤胃乳酸杆菌。这是关于具有TNF抑制活性的人类源萨氏乳酸杆菌分离株以及具有TNF刺激活性的人类本土物种瘤胃乳酸杆菌的首次报道。我们的数据表明这两种菌株作为免疫益生菌候选菌株具有潜在用途。
