Division of Clinical Microbiology, F68, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge, 141 86, Stockholm, Sweden,
Med Microbiol Immunol. 2015 Feb;204(1):131-5. doi: 10.1007/s00430-014-0384-8. Epub 2014 Dec 23.
DNA vaccination has historically failed to raise strong immune responses in humans. Recent delivery techniques such as the gene gun and in vivo electroporation (EP)/electrotransfer (ET) have completely changed the efficiency of DNA vaccines in humans. In vivo EP exerts multiple effects that contribute to its efficiency. The two central factors are most likely the increased DNA uptake due to the transient membrane destabilization, and the local tissue damage acting as an adjuvant. To date, several studies in humans have used in vivo EP/ET to deliver DNA. Some of these results have been quite promising with strong T cell responses and/or transient effects on the viral replication. This suggests that improved strategies of in vivo EP/ET can be a future way to deliver DNA in humans.
DNA 疫苗在临床上未能引起人体强烈的免疫反应。最近的基因枪和体内电穿孔（EP）/电转移（ET）等传递技术彻底改变了 DNA 疫苗在人体中的效率。体内 EP 产生了多种效果，有助于提高其效率。两个核心因素可能是由于瞬时膜不稳定导致的 DNA 摄取增加，以及作为佐剂的局部组织损伤。迄今为止，已有几项人体研究使用体内 EP/ET 来传递 DNA。其中一些结果非常有希望，因为它们产生了强烈的 T 细胞反应和/或对病毒复制的短暂影响。这表明，改进的体内 EP/ET 策略可能成为未来在人体中传递 DNA 的一种方式。
