液相色谱-质谱联用技术在临床生物化学中的原理与应用
Principles and applications of liquid chromatography-mass spectrometry in clinical biochemistry.
作者信息
Pitt James J
机构信息
VCGS Pathology, Murdoch Children's Research Institute, Royal Children's Hospital, Melbourne, Vic. 3052, Australia.
出版信息
Clin Biochem Rev. 2009 Feb;30(1):19-34.
Abstract
Liquid chromatography-mass spectrometry (LC-MS) is now a routine technique with the development of electrospray ionisation (ESI) providing a simple and robust interface. It can be applied to a wide range of biological molecules and the use of tandem MS and stable isotope internal standards allows highly sensitive and accurate assays to be developed although some method optimisation is required to minimise ion suppression effects. Fast scanning speeds allow a high degree of multiplexing and many compounds can be measured in a single analytical run. With the development of more affordable and reliable instruments, LC-MS is starting to play an important role in several areas of clinical biochemistry and compete with conventional liquid chromatography and other techniques such as immunoassay.
摘要
随着电喷雾电离（ESI）技术的发展，液相色谱 - 质谱联用（LC-MS）如今已成为一种常规技术，它提供了一个简单且稳定的接口。它可应用于多种生物分子，串联质谱（MS）和稳定同位素内标物的使用使得能够开发出高灵敏度和高精度的检测方法，不过需要进行一些方法优化以尽量减少离子抑制效应。快速扫描速度允许进行高度的多重分析，并且许多化合物可以在单次分析运行中进行测量。随着更经济实惠且可靠的仪器的发展，LC-MS开始在临床生物化学的多个领域发挥重要作用，并与传统液相色谱及其他技术（如免疫测定）展开竞争。
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