转录因子Oct4在膀胱癌细胞系T24中的表达及其对细胞生物学特性的影响。

Expression of transcription factor Oct4 in bladder cancer cell line T24 and its effects on the biological characteristics of the cells.

作者信息

Zhu Zhiqiang, Wen Jianguo, Zheng Xiangyu, Wang Daoxie, Wang Qingwei, Fan Changhui

机构信息

Department of Emergency Center and Pathology, The First Hospital of Zhengzhou University, Institute of Clinical Medical Research of Henan Universities, Zhengzhou, China.

出版信息

J Huazhong Univ Sci Technolog Med Sci. 2009 Feb;29(1):73-6. doi: 10.1007/s11596-009-0115-3. Epub 2009 Feb 18.

DOI:10.1007/s11596-009-0115-3

PMID:19224167

Abstract

The expression of octamer binding factor 4 (Oct4) gene in bladder cancer cell line T24 and its effects on the biological characteristics of the cells were investigated. RT-PCR and Western blot were employed to detect the expression of Oct4 in T24 cells. The changes of biological characteristics in T24 cells were analyzed before and after gene-silencing by Boyden chamber and MTT. The results showed that the expression of Oct4 gene was detectable in T24 cells by RT-PCR and Western blot. The expression of Oct4 gene and protein was down-regulated by siRNA, and average number of transwell cells in interference group, negative control group and blank control group was 101.40+/-4.56, 104.20+/-10.03 and 111.00+/-11.90, respectively. There was significant difference in the proliferation ability of the cells from 48 h, 72 h to 96 h after the interference by siRNA between interference group and negative group or blank control group (P<0.05). It was suggested that Oct4 gene was related with proliferation ability of T24 cells, but not with invasive capability.

摘要

研究了八聚体结合因子4（Oct4）基因在膀胱癌细胞系T24中的表达及其对细胞生物学特性的影响。采用RT-PCR和蛋白质印迹法检测T24细胞中Oct4的表达。通过Boyden小室和MTT分析基因沉默前后T24细胞生物学特性的变化。结果显示，RT-PCR和蛋白质印迹法可检测到T24细胞中Oct4基因的表达。siRNA可下调Oct4基因和蛋白的表达，干扰组、阴性对照组和空白对照组的穿膜细胞平均数分别为101.40±4.56、104.20±10.03和111.00±1.. 在siRNA干扰后48 h、72 h至96 h，干扰组与阴性组或空白对照组细胞的增殖能力存在显著差异（P<0.05）。提示Oct4基因与T24细胞的增殖能力有关，但与侵袭能力无关。