Hu Yong, Zhou Hongbo, Yu Zhengjun, Chen Huanchun, Jin Meilin
Division of Animal Pathogens, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070, Wuhan, Hubei, People's Republic of China.
Virus Genes. 2009 Apr;38(2):295-301. doi: 10.1007/s11262-009-0329-2. Epub 2009 Feb 18.
Based on the understanding that the analysis on unique short (US) region of duck enteritis virus (DEV) might contribute to the recognition of the molecular characterization and the evolution of DEV, in the study, a 5,121 bp fragment, which contained three genes encoding complete US10, unique short region open reading frame (SORF) 3, and US2 proteins, was amplified from the DEV (C-KCE) genome. The transcription orientation of the US10 and SORF3 was in a tail-to-tail way, and the SORF3 and US2 was the same. Potential core promoters and polyadenylation (poly(A)) sites were predicted for US10, SORF3, and US2 and further confirmed by polymerase chain reaction. Phylogenetic analysis for the three complete coding sequences showed that DEV was more closely related to avian herpesviruses, especially to Mardivirus, and should be classified to a separate genus of the Alphaherpesvirinae subfamily.
基于对鸭肠炎病毒（DEV）独特短区域（US）的分析可能有助于认识DEV分子特征和进化的理解，本研究从DEV（C-KCE）基因组中扩增出一个5121 bp的片段，该片段包含编码完整US10、独特短区域开放阅读框（SORF）3和US2蛋白的三个基因。US10和SORF3的转录方向为尾对尾，SORF3和US2的转录方向相同。对US10、SORF3和US2的潜在核心启动子和聚腺苷酸化（poly(A)）位点进行了预测，并通过聚合酶链反应进一步证实。对这三个完整编码序列的系统发育分析表明，DEV与禽疱疹病毒关系更密切，尤其是与马立克氏病毒属，应归为α疱疹病毒亚科的一个独立属。
