Batistini A P, Telles M P C, Bertoni B W, Coppede J S, Môro F V, França S C, Pereira A M S
Departamento de Biologia Aplicada à Agropecuária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP, Brazil.
Genet Mol Res. 2009 Jan 20;8(1):52-63. doi: 10.4238/vol8-1gmr538.
Catuaba (Anemopaegma arvense), a Bignoniaceae species endemic to Cerrado regions, shows anticancer properties and is widely used as a stimulant in traditional medicine. We evaluated the genetic diversity of seven populations found in the State of São Paulo, using random amplified polymorphic DNA markers. After optimization of the amplification reaction, 10 selected primers produced 70 reproducible bands, with 72.8% polymorphism. The greatest genetic diversity was observed within populations (71.72%). Variation estimates, theta(B) (0.2421) and Phi(ST) (0.283), obtained by inter- and intra-populational analysis of genetic variability of catuaba, indicated considerable population structure. However, the r value 0.346 (P = 0.099), calculated by the Mantel test, indicates that the genetic diversity among populations is not strongly structured in geographical space, although there appears to be a tendency towards structuring.
卡图巴（Anemopaegma arvense）是一种紫葳科植物，为塞拉多地区特有的物种，具有抗癌特性，在传统医学中被广泛用作兴奋剂。我们使用随机扩增多态性DNA标记评估了在圣保罗州发现的七个种群的遗传多样性。在优化扩增反应后，10条选定的引物产生了70条可重复的条带，多态性为72.8%。在种群内部观察到最大的遗传多样性（71.72%）。通过对卡图巴遗传变异性进行种群间和种群内分析获得的变异估计值theta(B)（0.2421）和Phi(ST)（0.283）表明存在相当大的种群结构。然而，通过Mantel检验计算得出的r值为0.346（P = 0.099），这表明尽管似乎存在一种结构化趋势，但种群间的遗传多样性在地理空间上并没有强烈的结构化。
