Departamento de Horticultura, Universidade Estadual Paulista "Júlio de Mesquita Filho", Botucatu, São Paulo, 18618-970, Brazil.
Departamento de Biotecnologia em Plantas Medicinais, Universidade de Ribeirão Preto-UNAERP, Ribeirão Preto, São Paulo, 14096-900, Brazil.
Mol Biol Rep. 2022 Sep;49(9):8617-8625. doi: 10.1007/s11033-022-07697-3. Epub 2022 Jul 22.
Pyrostegia venusta (Ker Gawl.) Miers occurs in threatened biodiversity hotspots of Cerrado and Atlantic forest biomes in Brazil and is used in traditional medicine to treat various respiratory and skin diseases.
This study (i) examined the genetic diversity and structure of six natural populations of P. venusta from different Brazilian regions using sequence-related amplified polymorphism (SRAP) markers; and (ii) compared the intra- and inter-populational levels of the bioactive component verbascoside using high-performance liquid chromatography. The population from Nova Mutum, Mato Grosso, presented the highest genetic variability (Nei index H = 0.2759; Shannon index I = 0.4170; 85.14% polymorphic loci), whereas the population from Araxá, Minas Gerais, presented the lowest genetic variability (H = 0.1811; I = 0.2820; 70.27% polymorphic loci). The intra-populational variability (79%) was significantly higher (p = 0.001) than the inter-populational variability (21%). The populations were clustered into two groups but their genetic differentiation was not associated with geographical origin (Mantel test, r = 0.328; p > 0.05). The verbascoside content significantly differed (p > 0.05) among the six populations and between the individuals from each population. The highest verbascoside levels (> 200 µg/mg extract) were detected in populations from Araxá and Serrana, while the lowest verbacoside levels were detected in populations from Paranaíta and Sinop.
This is the first report on the use of SRAP markers to analyze genetic variability in the family Bignoniaceae. Our findings shall help to better understand the genetic and chemical diversity of P. venusta populations, as well as provide useful information to select the most appropriate individuals to prepare phytomedicines.
美丽凌霄（Pyrostegia venusta（Ker Gawl.）Miers）存在于巴西塞拉多和大西洋森林生物群落的受威胁生物多样性热点地区，在传统医学中用于治疗各种呼吸道和皮肤疾病。
本研究（i）使用序列相关扩增多态性（SRAP）标记物，检测了来自巴西不同地区的六个美丽凌霄天然种群的遗传多样性和结构；（ii）比较了高效液相色谱法的内种群和种群间生物活性成分绒毛钩吻素的水平。来自马托格罗索州诺瓦穆图姆的种群表现出最高的遗传变异性（Nei 指数 H=0.2759；Shannon 指数 I=0.4170；85.14%多态性位点），而来自米纳斯吉拉斯州阿拉夏的种群表现出最低的遗传变异性（H=0.1811；I=0.2820；70.27%多态性位点）。种群内变异性（79%）显著高于种群间变异性（21%）（p=0.001）。种群聚为两个群，但它们的遗传分化与地理起源无关（Mantel 检验，r=0.328；p>0.05）。绒毛钩吻素的含量在六个种群之间以及每个种群的个体之间显著不同（p>0.05）。在阿拉夏和塞拉纳的种群中检测到最高的绒毛钩吻素水平（>200μg/mg 提取物），而在帕拉纳伊塔和新奥良的种群中检测到最低的绒毛钩吻素水平。
这是首次使用 SRAP 标记物分析美丽凌霄属家族遗传变异性的报道。我们的研究结果将有助于更好地理解美丽凌霄属种群的遗传和化学多样性，并为选择最合适的个体来制备植物药提供有用的信息。
