Sun Tao, Smallwood Rod, MacNeil Sheila
Department of Engineering Materials, Kroto Research Institute, University of Sheffield, Broad Lane, Sheffield S37HQ, UK.
J Mater Sci Mater Med. 2009 Jul;20(7):1483-93. doi: 10.1007/s10856-009-3703-7. Epub 2009 Feb 20.
A bioreactor system was developed using a series of fine mesh nickel grids as free standing scaffolds to investigate the behaviours of fibroblasts and keratinocytes in tissue culture. It was found that the mesh size of the suspended grids, but not of the grids that attached to tissue culture surface, had significant influences on cell behaviour and there was a maximum size for fibroblast to span within the defined culture period. Time lapse video microscopy demonstrated fibroblasts cultured on these grids initially migrated onto the struts but then worked together to fill in the voids between struts with a membranous sheet of tissue. In contrast keratinocytes barely migrated from the initial site of cell deposition and when they moved (to a modest extent) it was as an integrated sheet of cells. Similar results were observed when both types of cells were co-cultured in the system.
开发了一种生物反应器系统，使用一系列细网镍格栅作为独立支架，以研究成纤维细胞和角质形成细胞在组织培养中的行为。结果发现，悬浮格栅的网孔尺寸对细胞行为有显著影响，而附着在组织培养表面的格栅的网孔尺寸则没有，并且在规定的培养期内，成纤维细胞跨越的尺寸有一个最大值。延时视频显微镜显示，在这些格栅上培养的成纤维细胞最初迁移到支柱上，但随后共同协作，用一层膜状组织填充支柱之间的空隙。相比之下，角质形成细胞几乎没有从细胞沉积的初始位置迁移，当它们移动（程度有限）时，是以一片整合的细胞形式移动。当两种类型的细胞在该系统中共同培养时，也观察到了类似的结果。
