Hirose Shinichiro, Momosaki Sotaro, Hosoi Rie, Abe Kohji, Gee Antony, Inoue Osamu
Division of Health Sciences, Graduate School of Medicine, Osaka University, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan.
Ann Nucl Med. 2009 Feb;23(2):143-7. doi: 10.1007/s12149-008-0216-2. Epub 2009 Feb 19.
To clarify the role of N-methyl-D: -aspartate (NMDA) receptors upon [(14)C]acetate uptake in the rodent central nervous system (CNS), ibotenic acid (IBO) was infused into the right striatum of the rat brain.
Autoradiograms of [(14)C]acetate uptake in the brain for 2 h following the infusion of IBO (10 microg/microl) were obtained in both non-treated and MK-801 (1 mg/kg, i.v.) pretreated rats. The effect of MK-801 on [(14)C]acetate uptake in the normal rat brain was also studied.
Infusion of IBO significantly decreased [(14)C]acetate uptake in the infused side of the striatum. The expression of monocarboxylate transporter-1 was not altered, suggesting that the activity of tricarboxylic acid (TCA) cycle in glial cells might be depressed. Pretreatment with MK-801 completely blocked the decreasing effect of IBO on [(14)C]acetate uptake. MK-801 also increased [(14)C]acetate uptake in the whole brain of normal rats.
These results indicate the important roles of NMDA receptors on [(14)C]acetate uptake in the intact rat brain.
为阐明N-甲基-D-天冬氨酸（NMDA）受体在啮齿动物中枢神经系统（CNS）中对[¹⁴C]乙酸摄取的作用，将鹅膏蕈氨酸（IBO）注入大鼠脑的右侧纹状体。
在未处理和用MK-801（1mg/kg，静脉注射）预处理的大鼠中，获取注入IBO（10μg/μl）后2小时脑内[¹⁴C]乙酸摄取的放射自显影片。还研究了MK-801对正常大鼠脑内[¹⁴C]乙酸摄取的影响。
注入IBO显著降低了纹状体注入侧的[¹⁴C]乙酸摄取。单羧酸转运体-1的表达未改变，提示胶质细胞中三羧酸（TCA）循环的活性可能受到抑制。用MK-801预处理完全阻断了IBO对[¹⁴C]乙酸摄取的降低作用。MK-801还增加了正常大鼠全脑的[¹⁴C]乙酸摄取。
这些结果表明NMDA受体在完整大鼠脑内对[¹⁴C]乙酸摄取具有重要作用。
