Hosoi Rie, Okada Maki, Hatazawa Jun, Gee Antony, Inoue Osamu
School of Allied Health Sciences, Faculty of Medicine, Osaka University, Suita, Osaka, Japan.
J Cereb Blood Flow Metab. 2004 Feb;24(2):188-90. doi: 10.1097/01.WCB.0000098606.42140.02.
Fluorocitrate, a selective astrocytic toxin, was microinjected into the right striatum of rat brain, and the regional distribution of 14C-acetate was measured using autoradiography. A significant reduction (more than 80%) in 14C-acetate uptake over a 5-minute period was observed in the right striatum, compared with that in the left striatum (saline infused), 4 hours after fluorocitrate (1 nmol/microL) infusion. This effect was transient, and 14C-acetate uptake had almost returned to normal at 24 hours after the fluorocitrate infusion. In contrast, the regional blood flow in the striatum, as determined using 14C-iodoamphetamine, was significantly increased by the fluorocitrate infusion. The present observations indicate that 14C-acetate uptake might be a useful characteristic for examining astrocytic energy metabolism in the intact brain.
将选择性星形细胞毒素氟柠檬酸微量注射到大鼠脑的右侧纹状体中，并使用放射自显影法测量14C-乙酸盐的区域分布。在注入氟柠檬酸（1 nmol/μL）4小时后，观察到右侧纹状体在5分钟内14C-乙酸盐摄取量显著减少（超过80%），与左侧纹状体（注入生理盐水）相比。这种效应是短暂的，在注入氟柠檬酸24小时后，14C-乙酸盐摄取几乎恢复正常。相比之下，使用14C-碘安非他明测定的纹状体区域血流量因注入氟柠檬酸而显著增加。目前的观察结果表明，14C-乙酸盐摄取可能是检测完整大脑中星形细胞能量代谢的一个有用特征。
