Shimizu Yutaka, Nakamura Atsushi, Kishimura Hideki, Hara Akihiko, Watanabe Kazuhiko, Saeki Hiroki
Faculty of Fisheries Sciences, Hokkaido University, Hokkaido, Japan.
J Agric Food Chem. 2009 Mar 25;57(6):2314-9. doi: 10.1021/jf8031759.
Yolk protein extracts were prepared from four kinds of salmonid fish roes, and the proteins that reacted with IgE were screened by immunoblotting using sera from 20 patients allergic to chum salmon roe. IgE cross-reactivities among the salmonid yolk proteins were also investigated by competitive ELISA. The results were as follows: (1) The major protein components in salmonid roes were lipovitellin and beta'-component, which are subfragments of vitellogenin. (2) Most sera from the patients showed IgE reactivity to beta'-component in all yolk protein extracts, and some of them also reacted to lipovitellin heavy chain or its light chain. (3) Salmonid beta'-component showed high similarity (>90%) in the N-terminal amino acid sequence. (4) All of the salmonid yolk protein extracts inhibited the IgE reaction between patient sera and the chum salmon beta'-component. These findings indicate that the beta'-component in salmonid roe is a common major allergen with strong IgE cross-reactivity.
从四种鲑科鱼的鱼卵中制备了卵黄蛋白提取物，使用20名对大麻哈鱼鱼卵过敏患者的血清，通过免疫印迹法筛选与IgE反应的蛋白质。还通过竞争性ELISA研究了鲑科鱼卵黄蛋白之间的IgE交叉反应性。结果如下：（1）鲑科鱼鱼卵中的主要蛋白质成分是卵黄脂磷蛋白和β'-成分，它们是卵黄蛋白原的亚片段。（2）患者的大多数血清在所有卵黄蛋白提取物中均显示出对β'-成分的IgE反应性，其中一些还与卵黄脂磷蛋白重链或其轻链发生反应。（3）鲑科鱼的β'-成分在N端氨基酸序列中显示出高度相似性（>90%）。（4）所有鲑科鱼卵黄蛋白提取物均抑制患者血清与大麻哈鱼β'-成分之间的IgE反应。这些发现表明，鲑科鱼鱼卵中的β'-成分是一种常见的主要过敏原，具有很强的IgE交叉反应性。
