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对鲱鱼籽过敏患者进行鲱鱼籽与狭鳕鱼籽临床交叉反应性的血清学检查。

Serological examination for clinical cross-reactivity between salmon roe and pollock roe in patients with a salmon roe allergy.

作者信息

Okamoto Kaoru, Nakajima Yoichi, Yoshikawa Tetsushi, Kondo Yasuto

机构信息

Department of Pediatrics, School of Medicine, Fujita Health University, Toyoake, Aichi, Japan.

出版信息

Fujita Med J. 2022 May;8(2):52-58. doi: 10.20407/fmj.2021-004. Epub 2021 Aug 20.

DOI:10.20407/fmj.2021-004
PMID:35520294
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9069270/
Abstract

OBJECTIVES

Fish roe is a common allergen in Japan. We have previously reported that although immunoglobulin (IgE) from patients with salmon roe (SR) or pollock roe (PR) allergies cross-react, 70% of patients with SR allergy can consume PR without developing any symptoms. However, a correlation between clinical cross-reactivity and serological cross-reactivity remains to be demonstrated.

METHODS

Serum samples were collected from 15 patients with SR allergy who had consumed cooked PR previously. Among these volunteers, four had experienced immediate symptoms after consuming cooked PR, while the others had exhibited no symptoms of PR allergy. A competitive enzyme-linked immunosorbent assay (ELISA) was performed to analyze the serological cross-reactivity with SR and PR. Immunoblotting inhibition assays were performed using serum samples that had been pre-incubated with SR or PR extracts.

RESULTS

In ELISAs, binding to SR was inhibited by >50% when the serum samples from patients with both SR and PR allergies were pre-incubated with PR extract (=0.0256). In immunoblots, pre-incubation of serum samples with PR extract inhibited detection of the 16-kDa protein, which likely corresponds to the major SR allergen beta' component, significantly more for samples from patients with both SR and PR allergies (100%) than for samples from those with only an SR allergy (18.2%) (=0.011).

CONCLUSIONS

The superior competitive binding of the sera from patients with both SR and PR allergies to PR compared with that to SR may induce clinical cross-reactivity between SR and PR.

摘要

目的

鱼籽是日本常见的过敏原。我们之前曾报道，虽然来自鲑鱼籽（SR）或狭鳕鱼籽（PR）过敏患者的免疫球蛋白（IgE）会发生交叉反应，但70%的SR过敏患者可以食用PR而不出现任何症状。然而，临床交叉反应性与血清学交叉反应性之间的相关性仍有待证实。

方法

收集了15名曾食用过熟制PR的SR过敏患者的血清样本。在这些志愿者中，4人在食用熟制PR后出现了即时症状，而其他人未表现出PR过敏症状。进行了竞争性酶联免疫吸附测定（ELISA）以分析与SR和PR的血清学交叉反应性。使用预先与SR或PR提取物预孵育的血清样本进行免疫印迹抑制测定。

结果

在ELISA中，当SR和PR过敏患者的血清样本与PR提取物预孵育时，与SR的结合被抑制>50%（P = 0.0256）。在免疫印迹中，血清样本与PR提取物预孵育后，对于SR和PR过敏患者的样本，16 kDa蛋白（可能对应于主要的SR过敏原β'成分）的检测抑制更为显著（100%），而对于仅SR过敏患者的样本（18.2%）则不然（P = 0.011）。

结论

与SR相比，SR和PR过敏患者的血清对PR具有更强的竞争性结合，这可能会引发SR和PR之间的临床交叉反应性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25f6/9069270/3ecadeb9d246/fmj-8-052-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25f6/9069270/7cb6e4eeac31/fmj-8-052-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25f6/9069270/3ecadeb9d246/fmj-8-052-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25f6/9069270/7cb6e4eeac31/fmj-8-052-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25f6/9069270/3ecadeb9d246/fmj-8-052-g002.jpg

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Increased ratio of pollock roe-specific IgE to salmon roe-specific IgE levels is associated with a positive reaction to cooked pollock roe oral food challenge.鳕鱼子特异性 IgE 与鲑鱼子特异性 IgE 比值升高与煮熟的鳕鱼子口服食物挑战阳性反应相关。
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