含氮双膦酸盐处理的骨细胞的转录组和蛋白质组分析

Transcriptome and proteome analysis of osteocytes treated with nitrogen-containing bisphosphonates.

作者信息

Bivi Nicoletta, Bereszczak Jessica Z, Romanello Milena, Zeef Leo A H, Delneri Daniela, Quadrifoglio Franco, Moro Luigi, Brancia Francesco L, Tell Gianluca

机构信息

Department of Biomedical Sciences and Technologies, University of Udine, Udine, Italy, Shimadzu Research Laboratory (Europe), Manchester, UK.

出版信息

J Proteome Res. 2009 Mar;8(3):1131-42. doi: 10.1021/pr8005606.

DOI:10.1021/pr8005606

PMID:19226166

Abstract

We combined high-throughput screening of differential mRNAs with mass spectrometric characterization of proteins obtained from osteocytes untreated and treated with Risedronate. Microarray analysis revealed, upon treatment, a marked upregulation of messengers encoding zinc-proteins. MS analysis identified 84 proteins in the osteocytes proteome map. Risedronate affected the expression of 10 proteins, associated with cytoskeleton, stress-response and metabolism. Data validated using gel imaging in combination with the GLaD post digestion isotopic labeling method provide the molecular basis for understanding the role of bisphosphonates as antiapoptotic drugs for osteocytes.

摘要

我们将差异mRNA的高通量筛选与从未处理和用利塞膦酸盐处理的骨细胞中获得的蛋白质的质谱表征相结合。微阵列分析显示，处理后，编码锌蛋白的信使明显上调。质谱分析在骨细胞蛋白质组图谱中鉴定出84种蛋白质。利塞膦酸盐影响了10种与细胞骨架、应激反应和代谢相关的蛋白质的表达。结合凝胶成像和GLaD消化后同位素标记方法进行的数据验证为理解双膦酸盐作为骨细胞抗凋亡药物的作用提供了分子基础。