用于高通量筛选或小型化检测的细胞裂解方法。

Cell lysis methods for high-throughput screening or miniaturized assays.

作者信息

Lin Zhanglin, Cai Zhen

机构信息

Department of Chemical Engineering, Tsinghua University, Beijing, China.

出版信息

Biotechnol J. 2009 Feb;4(2):210-5. doi: 10.1002/biot.200800222.

DOI:10.1002/biot.200800222

PMID:19226554

Abstract

The widely and routinely used cell lysis techniques are still being evolved to address the technical needs of micro-scale sample processing, for example in high-throughput screening or miniaturized assays. This review focuses on several recently emerged mechanical, physical and biological methods designed for lysing cells on small scales for screening or analysis.

摘要

广泛且常规使用的细胞裂解技术仍在不断发展，以满足微观尺度样品处理的技术需求，例如在高通量筛选或小型化分析中。本综述重点关注了几种最近出现的用于小尺度细胞裂解以进行筛选或分析的机械、物理和生物学方法。