Jana B, Koszykowska M, Andronowska A
Division of Reproductive Endocrinology and Pathophysiology, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 0-747 Olsztyn, Poland.
Pol J Vet Sci. 2008;11(4):315-22.
The aim of the present study was to determine the effect of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta) and interleukin-6 (IL-6) on prostaglandin (PG)F2alpha and PGE2 secretion as well as on cyclooxygenase-2 (COX-2) protein expression in maternal placenta collected on days 25, 30 and 40 of pregnancy in pigs. Maternal placental slices were incubated for 16 h with TNF-alpha, IL-1beta and IL-6 and (1 or 10 ng/ml of medium) or two combinations of the three cytokines (1 or 10 ng/ml of each cytokine per combination). We demonstrated the stimulatory effect of TNF-alpha, IL-1beta and IL-6 and on PGF2alpha and PGE2 secretion by the porcine maternal placenta. The medium content of these PGs depended on the cytokine type, its concentration and day of pregnancy. Cytokine stimulation of PGE2 was more pronounced than that of PGF2alpha. Additionally, an increase in PGF2alpha and/or PGE2 secretion was usually associated with the augmentation of COX-2 protein expression. Our study shows that TNF-alpha, IL-1beta and IL-6 increase production of PGF2alpha and PGE2 by porcine maternal placenta from 25, 30 and 40 day of pregnancy. These results further confirm the possible role of cytokines in modulating secretion of PGs by maternal placenta during the first trimester of gestation.
本研究的目的是确定肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）对妊娠第25、30和40天采集的猪母体胎盘前列腺素（PG）F2α和PGE2分泌以及环氧合酶-2（COX-2）蛋白表达的影响。将母体胎盘切片与TNF-α、IL-1β和IL-6（1或10 ng/ml培养基）或三种细胞因子的两种组合（每种细胞因子组合1或10 ng/ml）孵育16小时。我们证明了TNF-α、IL-1β和IL-6对猪母体胎盘PGF2α和PGE2分泌的刺激作用。这些PGs的培养基含量取决于细胞因子类型、其浓度和妊娠天数。细胞因子对PGE2的刺激比对PGF2α更明显。此外，PGF2α和/或PGE2分泌的增加通常与COX-2蛋白表达的增加有关。我们的研究表明，TNF-α、IL-1β和IL-6可增加妊娠第25、30和40天猪母体胎盘PGF2α和PGE2的产生。这些结果进一步证实了细胞因子在妊娠早期调节母体胎盘PGs分泌中的可能作用。
