Gajecka M, Obremski K, Jakimiuk E, Skorska-Wyszyńska E, Zielonka L, Gajecki M
Division of Veterinary Prophylaxis and Feed Hygiene, Department of Veterinary Protection of Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland.
Pol J Vet Sci. 2008;11(4):363-6.
The present study deals with the influence of experimental ZEA mycotoxicosis on histopathological lesions in ovaries of bitches, which were administered zearalenone per os during anestrus phase for one hundred days. The experiment was performed on 9 sexually mature, clinically healthy bitches. The animals assigned into two experimental groups received zearalenone per os at two doses, 25 microg/kg b.w. and 50 microg/kg b.w., respectively: the bitches from control group received placebo per os. On the last day of zearalenone intoxication, the bitches were ovariohystorectomized. Histopathology and immunohistochemistry were performed. The study revealed that zearalenone and its metabolites caused profound regressive lesions: granular cells degeneration and atrophy. Numerous edemas and blood extravasations were also found. The intensity of these changes was significantly dose dependent. Furthermore, in ovarian cells and tissues of both experimental groups, no reaction for PCNA antigen was observed. In conclusion, zearalenone and its metabolites exerts unfavorable effects on the morphology of ovaries in bitches.
本研究探讨了实验性玉米赤霉烯酮中毒对母犬卵巢组织病理学损伤的影响，在发情间期对母犬经口给予玉米赤霉烯酮，持续100天。实验选用9只性成熟、临床健康的母犬。将动物分为两个实验组，分别经口给予两种剂量的玉米赤霉烯酮，即25微克/千克体重和50微克/千克体重：对照组母犬经口给予安慰剂。在玉米赤霉烯酮中毒的最后一天，对母犬进行卵巢子宫切除术。进行了组织病理学和免疫组织化学检查。研究发现，玉米赤霉烯酮及其代谢产物引起了严重的退行性病变：颗粒细胞变性和萎缩。还发现了大量水肿和血液外渗。这些变化的强度具有显著的剂量依赖性。此外，在两个实验组的卵巢细胞和组织中，均未观察到PCNA抗原反应。总之，玉米赤霉烯酮及其代谢产物对母犬卵巢形态产生不利影响。
