Gajecka M, Przybylska-Gornowicz B
Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland.
Pol J Vet Sci. 2012;15(4):711-20. doi: 10.2478/v10181-012-0108-1.
The objective of this study was to determine the effect of 42-day ZEN intoxication on the presence of Ca2+ in selected ovarian cells from beagle bitches, using the potassium pyroantimonate (PPA) method. Samples were collected from 30 clinically healthy, pre-pubertal, genetically homogeneous animals. The bitches were divided into three groups of 10 animals each: experimental group I--50 microg ZEN/kg BW (100% NOAEL) administered once daily per os; experimental group II--75 microg ZEN/kg BW (150% NOAEL) administered once dailyper os; control group--placebo containing no ZEN administered per os. An electron microscopic analysis revealed that cells died due to apoptosis, depending on the ZEN dose and the type of cells exposed to intoxication. Lower ZEN doses led to apoptosis-like changes in the cells. Cell death was a consequence of excess Ca2+ accumulation in the mitochondria, followed by cell dysfunction and a decrease in or the absence of mitochondrial metabolic activity in oocytes, follicle cells and interstitial cells in experimental bitches.
本研究的目的是采用焦锑酸钾（PPA）法，确定42天玉米赤霉烯酮中毒对比格犬选定卵巢细胞中Ca2+存在情况的影响。从30只临床健康、青春期前、基因同质的动物采集样本。这些母犬被分为三组，每组10只动物：实验组I——按50微克玉米赤霉烯酮/千克体重（100%未观察到有害作用水平）经口每日给药一次；实验组II——按75微克玉米赤霉烯酮/千克体重（150%未观察到有害作用水平）经口每日给药一次；对照组——经口给予不含玉米赤霉烯酮的安慰剂。电子显微镜分析显示，细胞因凋亡而死亡，这取决于玉米赤霉烯酮剂量以及接触中毒的细胞类型。较低剂量的玉米赤霉烯酮会导致细胞出现凋亡样变化。细胞死亡是线粒体中Ca2+过度积累的结果，随后实验母犬的卵母细胞、卵泡细胞和间质细胞出现细胞功能障碍以及线粒体代谢活性降低或缺失。
