Gordon L M, Sauerheber R D
Biochim Biophys Acta. 1977 Apr 1;466(1):34-43. doi: 10.1016/0005-2736(77)90206-1.
ESR spectra of egg lecithin dispersions labelled with 5-nitroxide stearic acid are recorded with a 50 G field sweep, and also with a new technique which "expands" the spectrum by (1) recording pairs of adjoining peaks with a smaller field sweep and (2) superposing the common peaks. The expansion technique improves the precision of the order parameters determined from the hyperfine splitting measurements, and may prove useful in future spin label membrane studies. Approximate order parameters are derived to describe the fluidity of fatty acid spin-labelled membranes in those cases where either the inner or outer hyperfine extrema are not well defined. The ability of these expressions to measure the fluidity of labelled egg lecithin disperions for the temperature range 14-42 degrees C is examined.
用50 G场扫描记录了用5-硝基硬脂酸标记的卵磷脂分散体的电子自旋共振（ESR）光谱，还用一种新技术记录了光谱，该技术通过（1）用较小的场扫描记录相邻峰对和（2）叠加共同峰来“扩展”光谱。这种扩展技术提高了由超精细分裂测量确定的序参数的精度，并且可能在未来的自旋标记膜研究中证明是有用的。在内部或外部超精细极值定义不明确的情况下，推导了近似序参数来描述脂肪酸自旋标记膜的流动性。研究了这些表达式在14-42摄氏度温度范围内测量标记卵磷脂分散体流动性的能力。
