大鼠肝膜亚组分的脂质流动性。
The lipid fluidity of rat liver membrane subfractions.
机构信息
Paterson Laboratories, Christie Hospital and Holt Radium Institute, Withington, Manchester M20 9BX, U.K.
出版信息
Biochem J. 1983 Sep 15;214(3):851-4. doi: 10.1042/bj2140851.
Abstract
- The lipid fluidity of three major rat liver plasma-membrane subfractions, as well as Golgi apparatus and endocytic fractions, was assessed with a fatty acid spin probe by using e.s.r. techniques. 2. The sinusoidal (blood-facing) plasma-membrane subfraction was the most fluid of the three plasma-membrane regions. Fractions originating from the bile-canalicular and contiguous (lateral) regions were most rigid. Endocytic fractions isolated (endosomes and diacytosomes) were of a similar fluidity to fractions originating from the sinusoidal plasma-membrane region. By far the most fluid fractions examined were derived from the Golgi-apparatus complex. 3. The three plasma-membrane subfractions each showed a different response to the bilayer-fluidizing effect of benzyl alcohol. 4. Arrhenius-type plots of the order parameter S and outer hyperfine splitting, 2T( parallel), identified lipid-phase separations in the plasma-membrane subfractions.
摘要
采用电子顺磁共振技术，利用脂肪酸旋转探针评估了大鼠肝三种主要质膜亚组分（高尔基体和内吞小体）以及内质网的脂流动性。
三种质膜区域中，窦状（面向血液）质膜亚组分最具流动性。来自胆小管和相邻（侧）区域的质膜亚组分最为僵硬。分离的内吞小体（内体和双核体）与来自窦状质膜区域的质膜亚组分具有相似的流动性。迄今为止，所检查的最具流动性的质膜亚组分源自高尔基体复合物。
三种质膜亚组分对苯甲醇的双层增溶作用的反应各不相同。
有序参数 S 和外超精细分裂 2T(平行) 的 Arrhenius 图确定了质膜亚组分中的脂质相分离。
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本文引用的文献
1
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J Cell Biol. 1982 Apr;93(1):97-102. doi: 10.1083/jcb.93.1.97.
2
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Biochim Biophys Acta. 1980 May 27;604(1):27-64. doi: 10.1016/0005-2736(80)90584-2.
3
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Biochem J. 1983 May 15;212(2):331-8. doi: 10.1042/bj2120331.
4
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