使用毛细管电泳法表征头孢羟氨苄与不同金属离子的相互作用。

Characterization of the interaction of cefadroxil with different metal ions using CE.

作者信息

Auda Sayed H, Mrestani Yahya, Ahmed Ahmed M S, Neubert Reinhard H H

机构信息

Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany.

出版信息

Electrophoresis. 2009 Mar;30(6):1066-70. doi: 10.1002/elps.200800436.

DOI:10.1002/elps.200800436

PMID:19229848

Abstract

The present study describes the application of CE to investigate the interaction between a cephalosporin, cefadroxil and different metal ions. This study aims at quantifying the interaction of cefadroxil with Zn(2+), Al(3+), Fe(3+), Cu(2+) and Co(2+) ions using the effective electrophoretic mobility as a parameter for calculation of the reaction association constant (K). For this purpose, the electrophoretic mobilities of cefadroxil at different metal ion concentrations in citrate buffer pH 3.6 were determined. A mathematical model to calculate the association constant between cefadroxil and metal ions was used. The results showed that a strong change in the cefadroxil electrophoretic mobility was observed by increasing the metal ion concentrations. The results revealed that by increasing the concentration of metal ions in the running buffer the degree of complexation increases too. On the basis of the results, the strength of the interaction of cefadroxil with the investigated metal ions follows the order Zn(2+)>Cu(2+)>Co(2+)>Fe(3+)>Al(3+). The association constant of the investigated reactions ranged from 451.57 to 1546.52 L/mmol. The results indicate that it is possible to characterize the interaction between cefadroxil and metal ions quantitatively using CE.

摘要

本研究描述了毛细管电泳（CE）在研究头孢菌素头孢羟氨苄与不同金属离子之间相互作用中的应用。本研究旨在以有效电泳迁移率作为计算反应缔合常数（K）的参数，对头孢羟氨苄与Zn(2+)、Al(3+)、Fe(3+)、Cu(2+)和Co(2+)离子的相互作用进行定量分析。为此，测定了在pH 3.6的柠檬酸盐缓冲液中不同金属离子浓度下头孢羟氨苄的电泳迁移率。使用了一个计算头孢羟氨苄与金属离子之间缔合常数的数学模型。结果表明，随着金属离子浓度的增加，头孢羟氨苄的电泳迁移率发生了显著变化。结果还显示，随着运行缓冲液中金属离子浓度的增加，络合程度也增加。根据结果，头孢羟氨苄与所研究金属离子相互作用的强度顺序为Zn(2+)>Cu(2+)>Co(2+)>Fe(3+)>Al(3+)。所研究反应的缔合常数范围为451.57至1546.52 L/mmol。结果表明，使用毛细管电泳可以定量表征头孢羟氨苄与金属离子之间的相互作用。