Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, 14049-900 Ribeirão Preto, SP, Brazil.
Microbiol Res. 2009;164(6):658-64. doi: 10.1016/j.micres.2008.12.005. Epub 2009 Feb 20.
The molecular mechanism that controls the response to phosphate shortage in Neurospora crassa involves four regulatory genes -nuc-2, preg, pgov, and nuc-1. Phosphate shortage is sensed by the nuc-2 gene, the product of which inhibits the functioning of the PREG-PGOV complex. This allows the translocation of the transcriptional factor NUC-1 into the nucleus, which activates the transcription of phosphate-repressible phosphatases. The nuc-2A mutant strain of N. crassa carries a loss-of-function mutation in the nuc-2 gene, which encodes an ankyrin-like repeat protein. In this study, we identified transcripts that are downregulated in the nuc-2A mutant strain. Functional grouping of these expressed sequence tags allowed the identification of genes that play essential roles in different cellular processes such as transport, transcriptional regulation, signal transduction, metabolism, protein synthesis, protein fate, and development. These results reveal novel aspects of the phosphorus-sensing network in N. crassa.
nuc-2、preg、pgov 和 nuc-1。nuc-2 基因感知磷酸盐饥饿，其产物抑制 PREG-PGOV 复合物的功能。这使得转录因子 NUC-1 易位到细胞核内，激活磷酸盐抑制性磷酸酶的转录。粗糙脉孢菌 nuc-2A 突变株携带 nuc-2 基因的功能丧失突变，该基因编码一个锚蛋白重复蛋白。在本研究中，我们鉴定了 nuc-2A 突变株中下调的转录本。对这些表达序列标签进行功能分组，鉴定了在不同细胞过程中发挥重要作用的基因，如运输、转录调控、信号转导、代谢、蛋白质合成、蛋白质命运和发育。这些结果揭示了粗糙脉孢菌磷感应网络的新方面。
