Kang S
Department of Biomolecular Chemistry, University of Wisconsin, Madison 53705.
Gene. 1993 Aug 25;130(2):259-64. doi: 10.1016/0378-1119(93)90428-6.
The NUC-1 regulatory protein directly controls the transcription of these genes and how the activity enzymes in Neurospora crassa. To understand how NUC-1 regulates the transcription of these genes and how the activity of NUC-1 is modulated by other regulatory proteins, two putative functional domains of NUC-1 were analysed: the DNA-binding domain and the regulatory domain. The DNA-binding activity of NUC-1 has not been directly demonstrated; however, results of deletion analysis, sequence analysis of the nuc-1 mutant alleles, and strong sequence similarity with the Saccharomyces cerevisiae PHO4 protein strongly suggest that the basic helix-loop-helix motif of NUC-1 forms a DNA-binding domain. Deletion and mutant analyses revealed that 39 amino acid (aa) residues (aa 463 to 501), or fewer, of NUC-1 are interacting with the negative regulatory factor(s), the PREG and/or PGOV proteins.
NUC-1调节蛋白直接控制这些基因的转录以及粗糙脉孢菌中酶的活性。为了了解NUC-1如何调节这些基因的转录以及NUC-1的活性如何被其他调节蛋白调控，对NUC-1的两个假定功能域进行了分析：DNA结合域和调节域。虽然尚未直接证明NUC-1的DNA结合活性；然而，缺失分析的结果、nuc-1突变等位基因的序列分析以及与酿酒酵母PHO4蛋白的高度序列相似性强烈表明，NUC-1的碱性螺旋-环-螺旋基序形成了一个DNA结合域。缺失和突变分析表明，NUC-1的39个氨基酸残基（第463至501位氨基酸）或更少的氨基酸残基与负调节因子PREG和/或PGOV蛋白相互作用。
