Department of Surgery and Anaesthesiology of Domestic Animals, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B9820 Merelbeke, Belgium.
Vet J. 2010 Apr;184(1):83-7. doi: 10.1016/j.tvjl.2009.01.006. Epub 2009 Feb 20.
The objective of this study was to evaluate an automated blood culture system for the isolation of microorganisms from infected equine synovial fluid (SF). Samples were collected from 220 severely inflamed synovial joints and classified as either presumably infected (group A: n=149) or not infected (group B: n=71), based on a combination of clinical history, clinical signs and cytological analysis of the SF. Samples were inoculated into blood culture bottles and after incubation were subcultured onto agar media to confirm the results and to facilitate full bacterial identification. Microorganisms were isolated from 107 group A samples (71.8%) and from three group B samples (4.2%). Overall, the detection system identified 117 bottles as positive and 103 as negative, including nine instrument-false-positives and two instrument-false-negatives. The median time-to-detection for Gram-positive bacteria, Gram-negative bacteria, and for fungi was 14.3 (interquartile range [I.R.] 10.0) h, 8.8 (I.R. 12.8) h, and 72.0 (range 60.8-74.8) h, respectively. It was concluded that culture of infected SF using the automated system combines the advantages of enrichment in specialised medium with the rapid detection of bacterial growth.
本研究旨在评估一种自动化血培养系统，以从感染的马属动物关节滑液（SF）中分离微生物。从 220 个严重炎症性关节采集样本，并根据临床病史、SF 的临床症状和细胞学分析，将其分为疑似感染（A 组：n=149）或未感染（B 组：n=71）。将样本接种到血培养瓶中，孵育后进行琼脂培养基的传代培养，以确认结果并促进细菌的充分鉴定。从 107 个 A 组样本（71.8%）和 3 个 B 组样本（4.2%）中分离出微生物。总体而言，检测系统鉴定出 117 个阳性瓶和 103 个阴性瓶，包括 9 个仪器假阳性和 2 个仪器假阴性。革兰氏阳性菌、革兰氏阴性菌和真菌的检测中位时间分别为 14.3（四分位距 [I.R.] 10.0）h、8.8（I.R. 12.8）h 和 72.0（范围 60.8-74.8）h。结论是，使用自动化系统培养感染性 SF 结合了在专用培养基中进行富集和快速检测细菌生长的优势。
