Martins Nelson, Bollinger Catherine, Harper Ruth M, Ribeiro Rui
IMAR-Instituto do Mar, Department of Zoology, University of Coimbra, Largo Marquês de Pombal, P-3004 517 Coimbra, Portugal. aa
Aquat Toxicol. 2009 Apr 9;92(2):104-12. doi: 10.1016/j.aquatox.2009.01.007. Epub 2009 Feb 23.
The increase in resistance to contaminants can result in the loss of genetic diversity of impacted populations. In this work, the effects of acid mine drainage (AMD) on the genetic diversity and structure of a historically exposed population of Daphnia longispina were evaluated using amplified fragment length polymorphism (AFLP) analysis. Individual sensitivity to acute copper exposure was determined in order to characterize the populations in terms of metal tolerance and in an attempt to identify possible contaminant indicative bands (CIB). No reduction in genetic diversity was found in the AMD impacted site population, in comparison to two reference populations. However, the analysis of molecular variance indicated a significant genetic differentiation from the two reference populations and a significant correlation between individual genetic distance and tolerance. The different average tolerance of individuals presenting one specific AFLP band indicated the existence of one putative CIB.
对污染物抗性的增加可能导致受影响种群遗传多样性的丧失。在本研究中，利用扩增片段长度多态性（AFLP）分析评估了酸性矿山排水（AMD）对历史上暴露的长刺溞种群的遗传多样性和结构的影响。测定了个体对急性铜暴露的敏感性，以便根据金属耐受性对种群进行特征描述，并试图识别可能的污染物指示条带（CIB）。与两个参考种群相比，在受AMD影响的位点种群中未发现遗传多样性降低。然而，分子方差分析表明，该种群与两个参考种群存在显著的遗传分化，且个体遗传距离与耐受性之间存在显著相关性。呈现一条特定AFLP条带的个体具有不同的平均耐受性，这表明存在一个推定的CIB。
