艾氏腹水癌细胞中环磷酸腺苷水平及乳酸生成的调节

Regulation of cyclic AMP level and lactic acid production in Ehrlich ascites tumor cells.

Graziani Y, Winikoff J, Chayoth R

Biochim Biophys Acta. 1977 Apr 27;497(2):499-506. doi: 10.1016/0304-4165(77)90207-0.

Abstract

Quercetin (3.3',4',5,7-pentahydroxy flavone) at the concentration of 10(-4) M, as well as 2-10(-2) M theophylline and 1.5 - 10(-4) M prostaglandin E2 caused maximal rise of cyclic AMP in Ehrlich ascites tumor cells. 2. No additional increase of cyclic AMP level in these cells was found when both quercetin (10(-4) M) and theophylline (2-10(-2) M) were present in the incubation medium, while combination of quercetin (10(-4) M) and prostaglandin E2 (1.5 - 10(-4) M) has a synergistic effect on the level of cyclic AMP. 3. Degradation of cyclic AMP by homogenate of Ehrlich ascites tumor cells was inhibited by both quercetin and theophylline. 4. Quercetin, and to a smaller but significant extent theophylline, inhibited the lactic acid production in Ehrlich ascites tumor cells while prostaglandin E2 did not change the glycolytic rate in these cells. No synergistic inhibitory effect on lactic acid production was found when combinations of quercetin and prostaglandin E2, quercetin and theophylline or prostaglandin E2 and theophylline were tested. 5. Treatment of Ehrlich ascites tumor cells with dextran sulfate abolished the inhibitory effect of quercetin on lactic acid production, while the effect of the bioflavonoid on cyclic AMP levels was not altered.

摘要

浓度为10⁻⁴ M的槲皮素（3,3',4',5,7 - 五羟基黄酮）以及2×10⁻² M的茶碱和1.5×10⁻⁴ M的前列腺素E2可使艾氏腹水癌细胞中的环磷酸腺苷（cAMP）水平达到最大升高。2. 当孵育培养基中同时存在槲皮素（10⁻⁴ M）和茶碱（2×10⁻² M）时，未发现这些细胞中的cAMP水平有额外升高，而槲皮素（10⁻⁴ M）与前列腺素E2（1.5×10⁻⁴ M）的组合对cAMP水平具有协同作用。3. 槲皮素和茶碱均抑制艾氏腹水癌细胞匀浆对cAMP的降解。4. 槲皮素以及程度较小但显著的茶碱抑制艾氏腹水癌细胞中的乳酸生成，而前列腺素E2并未改变这些细胞中的糖酵解速率。当测试槲皮素与前列腺素E2、槲皮素与茶碱或前列腺素E2与茶碱的组合时，未发现对乳酸生成有协同抑制作用。5. 用硫酸葡聚糖处理艾氏腹水癌细胞消除了槲皮素对乳酸生成的抑制作用，而该生物类黄酮对cAMP水平的影响未改变。