Slattery E, Taira H, Broeze R, Lengyel P
J Gen Virol. 1980 Jul;49(1):91-6. doi: 10.1099/0022-1317-49-1-91.
Conditions are described for the production of 0.3 to 0.7 NIH mouse reference standard units of interferon per cell from Ehrlich ascites tumour cells cultured as monolayers and induced by infection with Newcastle disease virus (NDV). Inclusion of theophylline (6 mM) in the medium increased the interferon yield three to four times. Cells infected with NDV started to lyse at about 15 p.i., but infected, theophylline-treated cells lysed only 24 p.i. Several other methylxanthines (e.g. theobromine, caffeine and isobutylmethylxanthine) when tested a concentrations similar to that of theophylline, did not boost interferon production. Dibutyryl cyclic AMP (10(-10) to 10(-2)M) did not substitute for theophylline in increasing interferon production, and, if used together with theophylline, did not cause further enhancement.
描述了从单层培养的艾氏腹水瘤细胞中生产每细胞0.3至0.7 NIH小鼠干扰素参考标准单位的条件，这些细胞通过新城疫病毒（NDV）感染诱导产生。培养基中加入茶碱（6 mM）可使干扰素产量提高三到四倍。感染NDV的细胞在感染后约15小时开始裂解，但经茶碱处理的感染细胞仅在感染后24小时裂解。当以与茶碱相似的浓度测试其他几种甲基黄嘌呤（如可可碱、咖啡因和异丁基甲基黄嘌呤）时，它们不会促进干扰素的产生。二丁酰环磷酸腺苷（10^(-10)至10^(-2)M）在增加干扰素产生方面不能替代茶碱，并且如果与茶碱一起使用，也不会导致进一步增强。
