Fedtsova N G
Ontogenez. 1991 May-Jun;22(3):237-44.
Undissociated tissue explants of the retina and retinal pigment epithelium (RPE) of 3,5-, 4-, 5- and 8-day-old chick embryos were cultured in vitro. After 7 days in culture, lentoids were observed in explants of either retina or RPE from 3,5-, 4- and 5-day-old embryos. As demonstrated by immunohistochemistry, these lentoids contained specific chick lens proteins (alpha-, beta- and delta-crystallins). No crystallin-containing cells were found in eye tissue explants from 8-day-old embryos. However, when 5-bromo-deoxyuridine (25 microM) was introduced into the medium at the beginning of culturing (for 12 h), large eosinophilic cells containing alpha-, beta- and delta-crystallins were detected in retinal explants of the 8-day old embryos. Thus, retina and RPE of 3,5-5-day-old chick embryos are capable of lens differentiation after explantation in vitro without dissociation into individual cells. This capacity is lost during development.
将3.5日龄、4日龄、5日龄和8日龄鸡胚的视网膜和视网膜色素上皮（RPE）的未分离组织外植体进行体外培养。培养7天后，在3.5日龄、4日龄和5日龄胚胎的视网膜或RPE外植体中观察到类晶状体。免疫组织化学显示，这些类晶状体含有特定的鸡晶状体蛋白（α-、β-和δ-晶状体蛋白）。在8日龄胚胎的眼组织外植体中未发现含晶状体蛋白的细胞。然而，当在培养开始时（培养12小时）将5-溴脱氧尿苷（25微摩尔）引入培养基中时，在8日龄胚胎的视网膜外植体中检测到含有α-、β-和δ-晶状体蛋白的大型嗜酸性细胞。因此，3.5至5日龄鸡胚的视网膜和RPE在体外培养后能够在不解离成单个细胞的情况下进行晶状体分化。这种能力在发育过程中丧失。
