Fedtsova N G, Barabanov V M
Ontogenez. 1978;9(6):609-15.
The ectoderm of oral regions from the chick embryos at the stage of 10 to 19 somites was cultivated in vitro and on chorioallantois in the complex with underlying tissues. In all the explants which, besides ectoderm, contained head gut and mesenchyme, lentoids and lenses formed within 6 days of in vitro cultivation. All specific antigens of chicken lens (alpha, beta- and delta-crystallins) were found in them by means of immunofluorescence. In the explants which contained diencephalon, besides single lentoids or lenses, adenohypophyses were found. The possibility of direct lens-inducing effect of the head gut endoderm on the ectoderm of oral region and the participation of diencephalon in this process are discussed.
将10至19体节阶段鸡胚口腔区域的外胚层与下层组织一起在体外和绒毛尿囊上进行培养。在所有除外胚层外还包含头肠和间充质的外植体中,体外培养6天内形成了类晶状体和晶状体。通过免疫荧光在其中发现了鸡晶状体的所有特异性抗原(α、β和δ晶状体蛋白)。在含有间脑的外植体中,除了单个类晶状体或晶状体外,还发现了腺垂体。讨论了头肠内胚层对口腔区域外胚层的直接晶状体诱导作用以及间脑在此过程中的参与情况。