Feng Qin-Zhong, Zhao Li-Xia, Yan Wei, Lin Jin-Ming, Zheng Zhi-Xia
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
J Hazard Mater. 2009 Aug 15;167(1-3):282-8. doi: 10.1016/j.jhazmat.2008.12.115. Epub 2008 Dec 31.
The molecularly imprinted bulk polymer with 2,4,6-trichlorophenol (2,4,6-TCP) as the template molecule and methylacrylic acid (MAA), ethylene glycol dimethacrylate (EGDMA) as functional monomer and the crosslinker, respectively, has been prepared and applied to the molecularly imprinted solid-phase extraction (MISPE) procedure for selective preconcentration of phenolic compounds from environmental water samples. Various parameters affecting the extraction efficiency of the polymer have been evaluated to optimize the selective preconcentration of the phenolic compounds from aqueous samples. The characteristics of the MISPE method were validated by HPLC. The recoveries ranged between 90% and 98% (RSD: 0.9-2.3%, n=3) for tap water, between 85% and 105% (RSD: 2.6-4.9%, n=3) for river water, between 78% and 98% (RSD: 2.6-5.4%, n=3) for sewage water fortified with 0.4 mg L(-1) of phenol, 4-chlorophenol (4-CP), 2,4-dichlorophenol (2,4-DCP), pentachlorophenol (PCP). It was demonstrated that this MISPE-HPLC method could be applied to direct preconcentration and determination of phenolic compounds in environmental water samples.
以2,4,6-三氯苯酚（2,4,6-TCP）为模板分子，甲基丙烯酸（MAA）、乙二醇二甲基丙烯酸酯（EGDMA）分别作为功能单体和交联剂，制备了分子印迹本体聚合物，并将其应用于分子印迹固相萃取（MISPE）过程，用于从环境水样中选择性富集酚类化合物。评估了影响该聚合物萃取效率的各种参数，以优化从水样中选择性富集酚类化合物的条件。采用高效液相色谱法（HPLC）对MISPE方法的特性进行了验证。自来水的回收率在90%至98%之间（相对标准偏差：0.9 - 2.3%，n = 3），河水的回收率在85%至105%之间（相对标准偏差：2.6 - 4.9%，n = 3），添加了0.4 mg L⁻¹苯酚、4-氯苯酚（4-CP）、2,4-二氯苯酚（2,4-DCP）、五氯苯酚（PCP）的污水的回收率在78%至98%之间（相对标准偏差：2.6 - 5.4%，n = 3）。结果表明，该MISPE - HPLC方法可用于直接富集和测定环境水样中的酚类化合物。
