Ye Xiujuan, Ng Tzi Bun
Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
Planta Med. 2009 Apr;75(5):550-6. doi: 10.1055/s-0029-1185312. Epub 2009 Feb 20.
A trypsin inhibitor with a molecular mass of about 19 kDa was isolated from seeds of Chinese black soybean Glycine max cv. "Small Glossy Black". It was isolated using a protocol that comprised ion exchange chromatography on Q-Sepharose, SP-Sepharose and DEAE-cellulose. It was adsorbed on all three ion exchangers. It inhibited trypsin with an IC(50) of 19 microM and chymotrypsin with an IC(50) of 14.3 microM. Its trypsin inhibitory activity was stable in the pH range pH 3-pH 13 and in the temperature range 0 degree C-60 degrees C. The trypsin inhibitor was inhibited by dithiothreitol (from 5 to 25 mM) in a dose-dependent manner. It exhibited an N-terminal sequence highly homologous to Kunitz-type trypsin inhibitors. It inhibited HIV-1 reverse transcriptase with an IC(50) of 0.16 microM, and suppressed proliferation of MCF-7 breast cancer cells with an IC(50) of 4.3 microM and HepG2 hepatoma cells with an IC(50) higher than 25 microM. The trypsin inhibitor lacked antifungal activity and mitogenic activity towards mouse splenocytes.
从中国黑豆（Glycine max cv. “Small Glossy Black”）种子中分离出一种分子量约为19 kDa的胰蛋白酶抑制剂。其分离采用了包括在Q-Sepharose、SP-Sepharose和DEAE-纤维素上进行离子交换色谱的方法。它能吸附在所有这三种离子交换剂上。它抑制胰蛋白酶的IC(50)为19 μM，抑制糜蛋白酶的IC(50)为14.3 μM。其胰蛋白酶抑制活性在pH 3至pH 13的范围内以及0℃至60℃的温度范围内均保持稳定。该胰蛋白酶抑制剂被二硫苏糖醇（5至25 mM）以剂量依赖性方式抑制。它表现出与Kunitz型胰蛋白酶抑制剂高度同源的N端序列。它抑制HIV-1逆转录酶的IC(50)为0.16 μM，抑制MCF-7乳腺癌细胞增殖的IC(50)为4.3 μM，抑制HepG2肝癌细胞增殖的IC(50)高于25 μM。该胰蛋白酶抑制剂对小鼠脾细胞缺乏抗真菌活性和促有丝分裂活性。
