Buergers Ralf, Hahnel Sebastian, Reischl Udo, Mueller Rainer, Rosentritt Martin, Handel Gerhard, Behr Michael
Department of Prosthetic Dentistry, University Medical Center, Regensburg, Germany.
Acta Odontol Scand. 2009;67(3):139-45. doi: 10.1080/00016350902729251.
This study aims at ranking various luting systems according to their susceptibility to adhering Streptococcus mutans and at evaluating the influence of incongruent mixing ratios on adhesion quantities.
Circular specimens measuring 8 mm in diameter were made of nine widely used dental cements -- three of them mixed in different ratios -- and then incubated with S. mutans. Adhering streptococci were quantified using a biofluorometric assay in combination with an automated plate reader for cell quantification. Surface roughness (R(a)) was determined by perthometer measurements.
Meron plus revealed the highest R(a) (0.90 microm) and glass the lowest R(a) (<0.01 microm). In regular cement mixtures, the highest mean fluorescence intensities indicated the presence of many viable bacteria [Meron Plus (35,533 relative fluorescence units (rfu)), Maxcem (13,374 rfu), and Panavia F 2.0 (11,701 rfu)]. Moderate fluorescence intensities were found in Harvard (4,171 rfu), Ketac cem (3,766 rfu), Durelon (3,276 rfu), Calibra (3,259), Rely X Unicem (4,358 rfu), and Bifix SE (3,102 rfu). A medium correlation between R(a) and S. mutans adhesion was found. Changes in regular cement proportions (powder/liquid and base/catalyst, respectively) had a significant influence on relative fluorescence intensities, which linearly increased with a higher proportion of liquid in Harvard and with a higher proportion of catalyst in Calibra and Maxcem.
Various luting systems revealed considerable differences in their potential to adhere S. mutans. Variations from recommended cement proportions led to significant changes in the amount of adhering streptococci.
本研究旨在根据不同粘结系统对变形链球菌的粘附敏感性进行排名，并评估不一致的混合比例对粘附量的影响。
用9种广泛使用的牙科水门汀制作直径8毫米的圆形标本，其中3种以不同比例混合，然后与变形链球菌一起培养。使用生物荧光测定法结合自动酶标仪对细胞进行定量，以量化粘附的链球菌。通过表面粗糙度仪测量表面粗糙度（R(a)）。
Meron plus的R(a)最高（0.90微米），玻璃的R(a)最低（<0.01微米）。在常规水门汀混合物中，最高的平均荧光强度表明存在许多活菌[Meron Plus（35,533相对荧光单位（rfu））、Maxcem（13,374 rfu）和Panavia F 2.0（11,701 rfu）]。在Harvard（4,171 rfu）、Ketac cem（3,766 rfu）、Durelon（3,276 rfu）、Calibra（3,259）、Rely X Unicem（4,358 rfu）和Bifix SE（3,102 rfu）中发现中等荧光强度。发现R(a)与变形链球菌粘附之间存在中等相关性。常规水门汀比例（分别为粉/液和基剂/催化剂）的变化对相对荧光强度有显著影响，在Harvard中，随着液体比例的增加，相对荧光强度呈线性增加，在Calibra和Maxcem中，随着催化剂比例的增加，相对荧光强度呈线性增加。
各种粘结系统在粘附变形链球菌的潜力方面存在显著差异。与推荐的水门汀比例不同会导致粘附链球菌数量的显著变化。
