Robinson M, Krco C J, Beito T G, David C S
Department of Immunology, Mayo Clinic, Rochester, MN 55905.
Parasite Immunol. 1991 Jul;13(4):391-404. doi: 10.1111/j.1365-3024.1991.tb00292.x.
Host antibody recognition of muscle larval (ML) antigens of Trichinella spiralis was examined. Monoclonal antibodies (MoAbs) to known host protective ML antigens have been produced in order to aid this examination. Eleven strains of mice with independent MHC haplotypes and seventeen T. spiralis infected human patients were all found to recognize the same three major antigens as the monoclonal antibodies; i.e., of mw 41, 46 and 55 kD. However all serum samples tested also recognized further ML antigens and this recognition varied with the individual or strain. This variation in antigen recognition also applied to the MoAb. Mutual inhibition studies demonstrated that even where the MoAb apparently recognized the same antigens, specific epitope recognition was disparate. Hence some of the major antigens recognized by hosts of T. spiralis, regardless of whether vaccinated or infected, correspond with antigens which have considerable host protective properties. There also appear to be a number of epitopes upon these antigens and the biological implications of this are discussed.
对旋毛虫肌幼虫（ML）抗原的宿主抗体识别情况进行了检测。为辅助此项检测，已制备出针对已知宿主保护性ML抗原的单克隆抗体（MoAb）。发现具有独立MHC单倍型的11株小鼠和17名旋毛虫感染的人类患者均识别与单克隆抗体相同的三种主要抗原，即分子量为41、46和55kD的抗原。然而，所有检测的血清样本也识别其他ML抗原，且这种识别因个体或菌株而异。抗原识别的这种差异也适用于单克隆抗体。相互抑制研究表明，即使单克隆抗体明显识别相同的抗原，其特异性表位识别也不同。因此，旋毛虫宿主（无论是否接种疫苗或感染）识别的一些主要抗原与具有相当宿主保护特性的抗原相对应。这些抗原上似乎也存在一些表位，并对其生物学意义进行了讨论。
