O'Sullivan J M, Sontam D M, Grierson R, Jones B
Institute of Molecular Biosciences, Massey University, Albany, New Zealand.
Yeast. 2009 Feb;26(2):125-38. doi: 10.1002/yea.1657.
The spatial organization of the chromosomes is crucial for gene expression and development. Inter- and intrachromosomal interactions form a crucial part of this epigenomic regulatory system. Here we use circular chromosome conformation capture-on-chip (4C) to identify interactions between repetitive and non-repetitive loci within the yeast genome. The interacting regions occur in non-randomly distributed clusters. Furthermore, the SIR2 histone deacetylase has opposing roles in the organization of the inter- or intrachromosomal interactions. These data establish a dynamic domain model for yeast genome organization. Moreover, they point to the repeated elements playing a central role in the dynamic organization of genome architecture.
染色体的空间组织对于基因表达和发育至关重要。染色体间和染色体内的相互作用构成了这个表观基因组调控系统的关键部分。在这里，我们使用环形染色体构象捕获芯片技术（4C）来识别酵母基因组中重复和非重复位点之间的相互作用。相互作用区域出现在非随机分布的簇中。此外，SIR2组蛋白脱乙酰酶在染色体间或染色体内相互作用的组织中具有相反的作用。这些数据建立了酵母基因组组织的动态结构域模型。此外，它们指出重复元件在基因组结构的动态组织中起着核心作用。
