Noir Sandra, Colby Thomas, Harzen Anne, Schmidt Jürgen, Panstruga Ralph
Max-Planck-Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, D-50829 Cologne, Germany.
Mol Plant Pathol. 2009 Mar;10(2):223-36. doi: 10.1111/j.1364-3703.2008.00524.x.
Conidiospores are the asexual propagation units of many plant-pathogenic fungi. In this article, we report an annotated proteome map of ungerminated conidiospores of the ascomycete barley powdery mildew pathogen, Blumeria graminis f.sp. hordei. Using a combination of two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-time-of-flight mass spectrometry, we have identified the proteins in 180 spots, which probably represent at least 123 distinct fungal gene products. Most of the identified proteins have a predicted function in carbohydrate, lipid or protein metabolism, indicating that the spore is equipped for the catabolism of storage compounds as well as for protein biosynthesis and folding on germination.
分生孢子是许多植物病原真菌的无性繁殖单位。在本文中，我们报道了子囊菌大麦白粉病病原菌——禾本科布氏白粉菌条形柄锈菌专化型未萌发分生孢子的注释蛋白质组图谱。通过二维聚丙烯酰胺凝胶电泳和基质辅助激光解吸电离飞行时间质谱联用技术，我们鉴定出了180个斑点中的蛋白质，这些斑点可能代表至少123种不同的真菌基因产物。大多数已鉴定的蛋白质在碳水化合物、脂质或蛋白质代谢方面具有预测功能，这表明孢子具备储存化合物的分解代谢能力以及萌发时蛋白质生物合成和折叠的能力。
