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丝状真菌的蛋白质组学

Proteomics of filamentous fungi.

作者信息

Kim Yonghyun, Nandakumar M P, Marten Mark R

机构信息

Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USA.

出版信息

Trends Biotechnol. 2007 Sep;25(9):395-400. doi: 10.1016/j.tibtech.2007.07.008. Epub 2007 Aug 3.

Abstract

Proteomic analysis, defined here as the global assessment of cellular proteins expressed in a particular biological state, is a powerful tool that can provide a systematic understanding of events at the molecular level. Proteomic studies of filamentous fungi have only recently begun to appear in the literature, despite the prevalence of these organisms in the biotechnology industry, and their importance as both human and plant pathogens. Here, we review recent publications that have used a proteomic approach to develop a better understanding of filamentous fungi, highlighting sample preparation methods and whole-cell cytoplasmic proteomics, as well as subproteomics of cell envelope, mitochondrial and secreted proteins.

摘要

蛋白质组学分析在这里被定义为对特定生物学状态下表达的细胞蛋白质进行全面评估,它是一种强大的工具,能够在分子水平上系统地理解各种事件。尽管丝状真菌在生物技术产业中普遍存在,并且作为人类和植物病原体具有重要意义,但对丝状真菌的蛋白质组学研究直到最近才开始出现在文献中。在这里,我们回顾了最近使用蛋白质组学方法来更好地理解丝状真菌的出版物,重点介绍了样品制备方法、全细胞胞质蛋白质组学,以及细胞膜、线粒体和分泌蛋白的亚蛋白质组学。

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