[Mechanism of action of ouabain and cyclic-3',5'-adenosine monophosphate on calcium ion transport in rat cardiac mitochondria].

Ouabain and cyclic AMP action on the Ca2+-accumulating capacity and the efflux of Ca2+ions from the loaded mitochondria of the rat heart was investigated by the tetracycline probe method. On the basis of the experimental results a conclusion was drawn that ouabain failed to act on these processes. Cyclic AMP didn't act on the Ca binding by the mitochondrial membrane; at the same time it induced rapid release of calcium from the loaded mitochondria.