Jankowski Jakob, Miething Andreas, Schilling Karl, Baader Stephan L
Institute of Anatomy, Anatomy and Cell Biology, University of Bonn, 53115 Bonn, Germany.
Cerebellum. 2009 Sep;8(3):277-90. doi: 10.1007/s12311-009-0093-9. Epub 2009 Feb 24.
Physiological cell death is crucial for matching defined cellular populations within the central nervous system. Whereas the time course of developmental cell death in the central nervous system is well analyzed, information about its precise spatial patterning is scarce. Yet, the latter one is needed to appraise its contribution to circuit formation and refinement. Here, we document that during normal cerebellar development, dying Purkinje cells were highly localized within the vermal midline and in a lobule specific, parasagittal pattern along the whole mediolateral axis. In addition, single hot spots of cell death localized to the caudal declive and ventral lobule IX within the posterolateral fissure. These hot spots of dying Purkinje cells partly overlapped with gaps within the Purkinje cell layer which supports the classification of different gaps based on histological and molecular criteria, i.e., midline gap, patchy gaps, and raphes. Areas characterized by a high incidence of Purkinje cell death and gaps colocalize with known molecular and functional boundaries within the cerebellar cortex. Physiological cell death can thus be considered to serve as an important regulator of cerebellar histogenesis.
生理性细胞死亡对于中枢神经系统中特定细胞群的匹配至关重要。虽然中枢神经系统中发育性细胞死亡的时间进程已得到充分分析，但关于其精确空间模式的信息却很少。然而，后者对于评估其对神经回路形成和精细化的贡献是必要的。在这里，我们记录到在正常小脑发育过程中，死亡的浦肯野细胞高度集中在蚓部中线以及沿整个内外侧轴呈小叶特异性的矢状旁模式。此外，细胞死亡的单个热点位于后外侧裂内的尾侧斜坡和腹侧小叶IX。这些死亡的浦肯野细胞热点部分与浦肯野细胞层内的间隙重叠，这支持了基于组织学和分子标准对不同间隙的分类，即中线间隙、斑片状间隙和缝际。以浦肯野细胞死亡高发和间隙为特征的区域与小脑皮质内已知的分子和功能边界共定位。因此，生理性细胞死亡可被视为小脑组织发生的重要调节因子。
