Saĭdzhafarova A O, Artemov G N, Karamysheva T V, Rubtsov N B, Stegniĭ V N
Genetika. 2009 Jan;45(1):59-63.
Using the method of microdissection of polytene chromosomes, followed by in situ hybridization, chromosomal localization of region-specific DNA probe from pericentic heterochromatin of chromosome 2L of Anopheles beklemishevi Stegnii et Kabanova was examined on polytene chromosomes of Anopheles atroparvus van Thiel, An. messeae Fall, and An. beklemishevi. DNA sequences homologous to the probe used were found in all species examined on chromosomes 2 and 3 in pericentric regions and in attachment regions. The exclusion were the attachment regions of chromosome XL in An. beklemishevi and An. messeae, and pericentric region of arm 2R in An. messeae. Pericentric a-heterochromatin of arm 2L in An. messeae and arm 3R in An. atroparvus also contained no sequences homologous to the DNA probe. The data obtained were compared with the earlier obtained data on localization of species-specific probe from the segment of chromosome 2R of An. atroparvus on chromosomes of An. artoparvus, An. messeae, and An. beklemishevi. The differences between the species in the sites of probes localization and fluorescence intensity revealed pointed to the existence of individual sequence associations in the regions of chromosomes attachment.
采用多线染色体显微切割方法，随后进行原位杂交，在嗜人按蚊（Anopheles atroparvus van Thiel）、梅氏按蚊（An. messeae Fall）和贝氏按蚊（An. beklemishevi）的多线染色体上，检测了来自贝氏按蚊染色体2L着丝粒周围异染色质的区域特异性DNA探针的染色体定位。在所检测的所有物种的染色体2和3的着丝粒区域和附着区域中，均发现了与所用探针同源的DNA序列。例外情况是贝氏按蚊和梅氏按蚊中染色体XL的附着区域，以及梅氏按蚊中臂2R的着丝粒区域。梅氏按蚊中臂2L的着丝粒α-异染色质和嗜人按蚊中臂3R的着丝粒α-异染色质也不包含与DNA探针同源的序列。将获得的数据与早期获得的关于嗜人按蚊染色体2R片段的物种特异性探针在嗜人按蚊、梅氏按蚊和贝氏按蚊染色体上定位的数据进行了比较。所揭示的探针定位位点和荧光强度在物种之间的差异表明，在染色体附着区域存在个体序列关联。
