Artemov Gleb N, Stegniy Vladimir N, Sharakhova Maria V, Sharakhov Igor V
Laboratory of Ecology, Genetics and Environmental Protection, Tomsk State University, 36 Lenin Avenue, 634050 Tomsk, Russia.
Department of Entomology, Fralin Life Science Institute, Institute and State University, Blacksburg, VA 24060, USA.
Insects. 2018 Sep 17;9(3):121. doi: 10.3390/insects9030121.
Anopheline mosquitoes are important vectors of human malaria. Next-generation sequencing opens new opportunities for studies of mosquito genomes to uncover the genetic basis of a transmission. Physical mapping of genome sequences to polytene chromosomes significantly improves reference assemblies. High-resolution cytogenetic maps are essential for anchoring genome sequences to chromosomes as well as for studying breakpoints of chromosome rearrangements and chromatin protein localization. Here we describe a detailed pipeline for the development of high-resolution cytogenetic maps using polytene chromosomes of malaria mosquitoes. We apply this workflow to the refinement of the cytogenetic map developed for
按蚊是人类疟疾的重要传播媒介。新一代测序为研究蚊子基因组以揭示传播的遗传基础带来了新机遇。将基因组序列物理定位到多线染色体上可显著改善参考组装。高分辨率细胞遗传图谱对于将基因组序列锚定到染色体以及研究染色体重排的断点和染色质蛋白定位至关重要。在此，我们描述了一种使用疟疾蚊子的多线染色体开发高分辨率细胞遗传图谱的详细流程。我们将此工作流程应用于为……开发的细胞遗传图谱的完善。
