牛磺酸卤胺与血红素加氧酶-1协同调节炎症并减轻氧化应激。

Taurine haloamines and heme oxygenase-1 cooperate in the regulation of inflammation and attenuation of oxidative stress.

作者信息

Marcinkiewicz Janusz, Walczewska Maria, Olszanecki Rafał, Bobek Małgorzata, Biedroń Rafał, Dulak Józef, Józkowicz Alicja, Kontny Ewa, Maślinski Włodzimierz

机构信息

Chair of Immunology, Institute of Rheumatology, Poland.

出版信息

Adv Exp Med Biol. 2009;643:439-50. doi: 10.1007/978-0-387-75681-3_46.

DOI:10.1007/978-0-387-75681-3_46

PMID:19239176

Abstract

Taurine chloramine (TauCl) and Taurine bromamine (TauBr), products of the neutrophil myeloperoxidase halide system, exert anti-inflammatory properties. They inhibit the production of a variety of inflammatory mediators, such as prostaglandin E2 (PGE2), nitric oxide (NO) and proinflammatory cytokines. Heme oxygenase-1 (HO-1), a stress inducible enzyme, degrades heme to biliverdin, free iron and carbon monoxide (CO), which are involved in the anti-inflammatory and antioxidant actions of HO-1. Recently we have demonstrated that taurine haloamines induce the expression of HO-1 in inflammatory cells. In this study we examined whether HO-1 participates in taurine haloamines-mediated suppression of proinflammatory cytokine production. We have shown that TauCl/TauBr and CO inhibit the production of TNF-alpha, IL-12 and IL-6, in a similar dose-dependent manner. However, the suppressor activity of TauCl was not altered in HO-1 deficient mice. Therefore, HO-1 and TauCl may independently regulate the production of proinflammatory cytokines. We suggest that TauCl and TauBr provide a link between the two antioxidant systems: the cysteine pathway and the heme oxygenase system.

摘要

牛磺酸氯胺（TauCl）和牛磺酸溴胺（TauBr）是中性粒细胞髓过氧化物酶卤化物系统的产物，具有抗炎特性。它们可抑制多种炎症介质的产生，如前列腺素E2（PGE2）、一氧化氮（NO）和促炎细胞因子。血红素加氧酶-1（HO-1）是一种应激诱导酶，可将血红素降解为胆绿素、游离铁和一氧化碳（CO），这些物质参与了HO-1的抗炎和抗氧化作用。最近我们证明，牛磺酸卤胺可诱导炎症细胞中HO-1的表达。在本研究中，我们检测了HO-1是否参与牛磺酸卤胺介导的促炎细胞因子产生的抑制作用。我们发现，TauCl/TauBr和CO以相似的剂量依赖性方式抑制肿瘤坏死因子-α（TNF-α）、白细胞介素-12（IL-12）和白细胞介素-6（IL-6）的产生。然而，在HO-1缺陷小鼠中，TauCl的抑制活性并未改变。因此，HO-1和TauCl可能独立调节促炎细胞因子的产生。我们认为，TauCl和TauBr在两个抗氧化系统之间建立了联系：半胱氨酸途径和血红素加氧酶系统。