Olszanecki R, Kurnyta M, Biedroń R, Chorobik P, Bereta M, Marcinkiewicz J
Chair of Pharmacology, Jagiellonian University Medical College, Kraków, Poland.
Amino Acids. 2008 Aug;35(2):359-64. doi: 10.1007/s00726-007-0609-x. Epub 2007 Dec 21.
Taurine chloramine (TauCl) and taurine bromamine (TauBr), products of myeloperoxidase halide system, exert anti-inflammatory properties. TauCl was demonstrated to inhibit the production of a variety of pro-inflammatory mediators including cyclooxygenase-2 (COX-2) dependent production of prostaglandin E(2) (PGE(2)). Recently we have demonstrated that both major leukocyte haloamines, TauCl and TauBr, induced expression of HO-1 in non-activated and LPS-activated J774.2 macrophages. In this study, we have shown that TauCl and TauBr, at non-cytotoxic concentrations, inhibited the production of (PGE(2)) without altering the expression of COX-2 protein, in LPS/IFN-gamma stimulated J774.2 cells. The inhibitory effect of TauCl and TauBr was reversed by chromium III mesoporhyrin (CrMP), an inhibitor of HO-1 activity. Our data suggest that HO-1 might participate in anti-inflammatory effects of TauCl/TauBr possibly by inhibition of COX-2 activity and decrease of PGE(2) production.
髓过氧化物酶卤化物系统的产物氯胺牛磺酸（TauCl）和溴胺牛磺酸（TauBr）具有抗炎特性。已证实TauCl可抑制多种促炎介质的产生，包括环氧合酶-2（COX-2）依赖性的前列腺素E2（PGE2）的产生。最近我们证明，两种主要的白细胞卤胺，即TauCl和TauBr，均可在未激活和LPS激活的J774.2巨噬细胞中诱导血红素加氧酶-1（HO-1）的表达。在本研究中，我们发现，在LPS/γ干扰素刺激的J774.2细胞中，非细胞毒性浓度的TauCl和TauBr可抑制PGE2的产生，而不改变COX-2蛋白的表达。HO-1活性抑制剂三价铬中卟啉（CrMP）可逆转TauCl和TauBr的抑制作用。我们的数据表明，HO-1可能通过抑制COX-2活性和减少PGE2产生参与TauCl/TauBr的抗炎作用。
