Laoong-u-thai Yanisa, Zhao Baoping, Phongdara Amornrat, Ako Harry, Yang Jinzeng
Department of Human Nutrition, Food and Animal Sciences, University of Hawaii at Manoa, Honolulu, HI 96822, USA.
Int J Biol Sci. 2009;5(2):205-14. doi: 10.7150/ijbs.5.205. Epub 2009 Feb 19.
Small ubiquitin-like modifiers (SUMO) work in a similar way as ubiquitin to alter the biological properties of a target protein by conjugation. A shrimp SUMO cDNA named LvSUMO-1 was identified in Litopenaeus vannamei. LvSUMO-1 cDNA contains a coding sequence of 282 nucleotides with untranslated regions of 37 bp at 5'-end and 347 bp at 3'-end, respectively. The deduced 93 amino acids exhibit 83% identity with the Western Honeybee SUMO-1, and more than 65% homologies with human and mouse SUMO-1. LvSUMO-1 mRNA is expressed in most L. vannamei tissues with the highest level in hepatopancrease. The mRNA expression of LvSUMO-1 over development stages in L. Vammamei is distinguished by a low level in nauplius stage and relatively high level in postlarva stage with continuous expression until juvenile stage. The LvSUMO-1 protein and its conjugated proteins are detected in both cytoplasm and nucleus in several tissues. Interestingly, LvSUMO-1 mRNA levels are high in abdominal muscle during the premolt stage, wherein it has significant activities of protein degradation, suggesting its possible role in the regulation of shrimp muscle protein degradation.
小泛素样修饰物（SUMO）的作用方式与泛素类似，通过缀合作用改变靶蛋白的生物学特性。在凡纳滨对虾中鉴定出一种名为LvSUMO-1的对虾SUMO cDNA。LvSUMO-1 cDNA包含一个282个核苷酸的编码序列，其5'端非翻译区为37 bp，3'端非翻译区为347 bp。推导的93个氨基酸与西方蜜蜂的SUMO-1具有83%的同一性，与人类和小鼠的SUMO-1具有超过65%的同源性。LvSUMO-1 mRNA在凡纳滨对虾的大多数组织中表达，在肝胰腺中表达水平最高。凡纳滨对虾发育阶段中LvSUMO-1的mRNA表达在无节幼体阶段水平较低，在仔虾阶段相对较高，并持续表达直至幼体阶段。在多个组织的细胞质和细胞核中均检测到LvSUMO-1蛋白及其缀合蛋白。有趣的是，在蜕皮前期，腹部肌肉中的LvSUMO-1 mRNA水平较高，此时其具有显著的蛋白质降解活性，表明其可能在对虾肌肉蛋白质降解的调节中发挥作用。
