[11个犬STR基因座的遗传多态性]
[Genetic polymorphism of eleven canine STR loci].
作者信息
Du Wei-An, Xiong Yong-Hua, Ye Jun-Hua, Yang Qian-Yong
机构信息
Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China.
出版信息
Fa Yi Xue Za Zhi. 2008 Dec;24(6):425-8.
OBJECTIVE
To investigate the polymorphism of 11 canine STR loci.
METHODS
A fluorescent multiplex system with 11 STR loci (PEZ1, PEZ2, PEZ3, PEZ5, PEZ6, PEZ8, PEZ12, FH2010, FH2054, FH2132 and FH2611) was constructed independently and performed to amplify 105 samples from dogs. The character of these loci was analyzed with the PCR data.
RESULTS
The distributions of genotypes and allele frequencies of 11 STR loci were obtained. The total power of discrimination for the 11 loci in canine population was 0.9999999 and the cumulative probability of exclusion was 0.9330621. The observed heterozygosity and polymorphism information content (PIC) were 0.502 and 0.640, respectively.
CONCLUSION
Each of the eleven canine STR loci has a high genetic polymorphism and can be applied for the parentage testing and individual identification. The fluorescent multiplex system is a reliable method in forensic application.
目的
研究11个犬类短串联重复序列（STR）位点的多态性。
方法
独立构建一个包含11个STR位点（PEZ1、PEZ2、PEZ3、PEZ5、PEZ6、PEZ8、PEZ12、FH2010、FH2054、FH2132和FH2611）的荧光多重体系，并对105份犬类样本进行扩增。利用PCR数据对这些位点的特征进行分析。
结果
获得了11个STR位点的基因型和等位基因频率分布。这11个位点在犬类群体中的总鉴别力为0.9999999，累积排除概率为0.9330621。观察到的杂合度和多态信息含量（PIC）分别为0.502和0.640。
结论
11个犬类STR位点均具有高度的遗传多态性，可用于亲子鉴定和个体识别。该荧光多重体系是法医应用中的一种可靠方法。
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