发育中灵长类动物睾丸组织中表达的微小RNA的微阵列分析。
Microarray profiling of microRNAs expressed in testis tissues of developing primates.
作者信息
Yan Naihong, Lu Yilu, Sun Huaqin, Qiu Weimin, Tao Dachang, Liu Yunqiang, Chen Huijiao, Yang Yuan, Zhang Sizhong, Li Xiang, Ma Yongxin
机构信息
Department of Medical Genetics, West China Hospital, Division of Human Morbid Genomics, State Key Laboratory of Biotherapy, Sichuan University, Chengdu, People's Republic of China.
出版信息
J Assist Reprod Genet. 2009 Apr;26(4):179-86. doi: 10.1007/s10815-009-9305-y. Epub 2009 Feb 26.
PURPOSE
MicroRNAs (miRNAs) are small non-coding RNA molecules that have been identified as potent regulators of gene expression. Recent studies indicate that miRNAs are involved in mammalian spermatogenesis but the mechanism of regulation is largely unknown.
METHODS
miRNA microarray was employed to compare miRNA expression profiles of testis tissues from immature rhesus monkey (Sample IR), mature rhesus monkey (Sample MR), and mature human (Sample MH). Real-time RT-PCR was used to confirm the changed miRNAs.
RESULTS
Twenty-six miRNAs were shared by samples IR/MR and IR/MH with differential expression patterns greater than three-fold difference. PicTar and TargetScan prediction tools predicted a number of target mRNAs, and some of these target genes predicted by miRNAs have been shown to associate with spermatogenesis.
CONCLUSIONS
Our results indicate that miRNAs are extensively involved in spermatogenesis and provide additional information for further studies of spermatogenetic mechanisms.
目的
微小RNA（miRNA）是一类小的非编码RNA分子，已被确认为基因表达的强效调节因子。近期研究表明，miRNA参与哺乳动物精子发生过程，但调控机制大多未知。
方法
采用miRNA微阵列比较未成熟恒河猴（样本IR）、成熟恒河猴（样本MR）和成熟人类（样本MH）睾丸组织的miRNA表达谱。运用实时逆转录聚合酶链反应（RT-PCR）来确认表达发生变化的miRNA。
结果
样本IR/MR和IR/MH共有26种miRNA，其差异表达模式差异超过三倍。PicTar和TargetScan预测工具预测了许多靶mRNA，其中一些miRNA预测的靶基因已被证明与精子发生有关。
结论
我们的结果表明，miRNA广泛参与精子发生，并为进一步研究精子发生机制提供了更多信息。
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