Hansen Alexandar L, Nikolova Evgenia N, Casiano-Negroni Anette, Al-Hashimi Hashim M
Department of Chemistry and Biophysics, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan 48109, USA.
J Am Chem Soc. 2009 Mar 25;131(11):3818-9. doi: 10.1021/ja8091399.
We present an off-resonance carbon R(1rho) NMR experiment utilizing weak radiofrequency fields and selective polarization transfers for quantifying chemical-exchange processes in nucleic acids. The experiment extends the range of accessible time scales to approximately 10 ms, and its time-saving feature makes it possible to thoroughly map out dispersion profiles and conduct measurements at natural abundance. The experiment unveiled microsecond-to-millisecond exchange dynamics in a uniformly labeled A-site rRNA and in unlabeled, damaged DNA that would otherwise be difficult to characterize by conventional methods.
我们展示了一种利用弱射频场和选择性极化转移来量化核酸中化学交换过程的非共振碳R(1rho)核磁共振实验。该实验将可及的时间尺度范围扩展到约10毫秒，其节省时间的特性使得全面绘制色散图谱并在天然丰度下进行测量成为可能。该实验揭示了均匀标记的A位点rRNA和未标记的受损DNA中从微秒到毫秒的交换动力学，而这些用传统方法很难进行表征。
