Ganpath N, Prestayko A W, Busch H
Cancer Res. 1977 May;37(5):1290-300.
As part of a continuing comparison of nuclear proteins of tumors and other tissues, 32P-labeled nuclear proteins were extracted successively with 0.15 and 0.35 m NaC1 from the nuclei of normal, regenerating, and thioacetamide-treated rat liver as well as Novikoff hepatoma 3 hr after injection of 32Pi into rats. Separation of proteins of these fractions with aqueous phenol was carried out before two-dimensional electrophoresis on polyacrylamide gels. By autoradiography many common spots were found, but four 32P-labeled protein spots, CU', C13p, C21p, and CMp, were found in the Novikoff hepatoma and not in the various liver samples studied. Two spots, B6 and B10, were found in the liver patterns and not in the tumor. Sot B33 was very dense in regenerating liver but was only a faint spot in thioacetamide-treated liver. The greater density of Spots CU', C13p, C21p, and CMp in the tumor patterns is consistent with the increased density reported earlier for spots of the C-region of a variety of tumors.
作为肿瘤和其他组织核蛋白持续比较研究的一部分，在给大鼠注射³²P₁ 3小时后，从正常、再生以及硫代乙酰胺处理的大鼠肝脏细胞核以及诺维科夫肝癌细胞核中，先后用0.15和0.35m NaCl提取³²P标记的核蛋白。在聚丙烯酰胺凝胶上进行二维电泳之前，先用苯酚水溶液分离这些组分的蛋白质。通过放射自显影发现了许多共同的斑点，但在诺维科夫肝癌中发现了四个³²P标记的蛋白质斑点，即CU'、C13p、C21p和CMp，而在所研究的各种肝脏样本中未发现。在肝脏图谱中发现了两个斑点B6和B10，而在肿瘤中未发现。斑点Sot B33在再生肝脏中非常密集，但在硫代乙酰胺处理的肝脏中只是一个 faint 斑点。肿瘤图谱中斑点CU'、C13p、C21p和CMp的密度更高，这与之前报道的各种肿瘤C区斑点密度增加一致。 （注：原文中“faint”未翻译完整，推测是“微弱的”之意，可根据实际情况调整。）
