Capetanaki Y G, Alonso A
Nucleic Acids Res. 1980 Jul 25;8(14):3193-214. doi: 10.1093/nar/8.14.3193.
Polysomal and nuclear poly(A)-containing RNA of normal rat liver and Novikoff hepatoma cells have been compared by cDNA.RNA hybridization kinetics. Homologous hybridization reactions revealed at total kinetic complexity of about 1.6 X 10(10) and 1.38 X 10(10) daltons for liver and Novikoff mRNA respectively. The high abundance component present in liver cannot be detected in Novikoff. It was found from heterologous reactions that about 30% by weight of mRNA sequences are specific to liver. Determination of the nuclear poly(A)-containing RNA complexities revealed that about 5.5% and 4% of the haploid genome is expressed in the liver and Novikoff respectively. In a heterologous reaction, up to 30% of the liver cDNA failed to form hybrids with Novikoff nuclear RNA. Cross hybridizations have further revealed abundance shifts in both nuclear and polysomal RNA populations. Some sequences abundant in liver are less abundant in Novikoff and some rare liver sequences are relatively abundant in Novikoff.
已通过cDNA-RNA杂交动力学比较了正常大鼠肝脏和诺维科夫肝癌细胞的多核糖体和细胞核中含poly(A)的RNA。同源杂交反应显示，肝脏和诺维科夫mRNA的总动力学复杂度分别约为1.6×10¹⁰道尔顿和1.38×10¹⁰道尔顿。在诺维科夫细胞中无法检测到肝脏中存在的高丰度成分。从异源反应中发现，约30%（按重量计）的mRNA序列是肝脏特有的。对细胞核中含poly(A)的RNA复杂度的测定表明，单倍体基因组中分别约有5.5%和4%在肝脏和诺维科夫细胞中表达。在异源反应中，高达30%的肝脏cDNA无法与诺维科夫细胞核RNA形成杂交体。交叉杂交进一步揭示了细胞核和多核糖体RNA群体中的丰度变化。一些在肝脏中丰富的序列在诺维科夫细胞中较少，而一些在肝脏中罕见的序列在诺维科夫细胞中相对丰富。
