Sacakli Pinar, Tuncer Sakir Doğan, Koksal Bekir Hakan, Selcuk Zehra, Bugdayci Kadir Emre
Ankara University, Faculty of Veterinary Medicine, Department of Animal Nutrition, Diskapi, 06110 Ankara, Turkey.
Dtsch Tierarztl Wochenschr. 2009 Feb;116(2):64-9.
A high ruminal protein degradation is especially undesirable in high producing dairy cows and beef cattle, since these animals have high requirements of protein and the amount of protein that is not degraded by ruminal microorganisms and therefore enters the small intestine (so-called UDP = undegraded protein) is crucial. In this study, Merino rams with ruminal fistulas were used (n = 4) to determine the effect of glucose treatment of soybean meal (SBM), full fat soybean (FFSB) and soybean seed (SBS) on the ruminal degradability of dry matter (DM) and crude protein (CP). All these feedstuffs were grinded, diluted with water and heated (2 h; 100 degrees C) either without or with supplementation of 2 or 3% glucose (20 or 30 g glucose per kg dry feed). For the determination of the ruminal degradability nylon bag technique was used (incubation times: 2-48 h). In general ruminal degradability of protein in all three soy products was reduced by glucose treatment. In FFSB CP disappearance started to decrease after 8 h of incubation, whereas in SBM (+ 3% glucose) and SBS (+ 2 or 3% glucose) reduction of CP disappearance were time-independent. Further, effective degradability (Pe) of DM and CP of all feedstuffs were significantly reduced by glucose treatment, with the reduction being more pronounced when the glucose level was increased. When adding 3% of glucose, the Pe for CP was reduced by 6 (FFSB), 16 (SBM) and 18% (SBS), respectively. Explanation for the observed effects of a glucose supplementation (incl. water and heat treatment) is the reductive activity of glucose within these conditions.
在高产奶牛和肉牛中,瘤胃蛋白质高降解率尤其不可取,因为这些动物对蛋白质的需求量很大,未被瘤胃微生物降解从而进入小肠的蛋白质数量(即所谓的UDP = 未降解蛋白质)至关重要。在本研究中,使用了装有瘤胃瘘管的美利奴公羊(n = 4)来测定葡萄糖处理对豆粕(SBM)、全脂大豆(FFSB)和大豆种子(SBS)的干物质(DM)和粗蛋白(CP)瘤胃降解率的影响。所有这些饲料原料均经研磨、加水稀释并加热(2小时;100摄氏度),加热时不添加或添加2%或3%的葡萄糖(每千克干饲料添加20或30克葡萄糖)。采用尼龙袋技术测定瘤胃降解率(培养时间:2 - 48小时)。总体而言,葡萄糖处理降低了所有三种大豆产品中蛋白质的瘤胃降解率。在FFSB中,CP消失率在培养8小时后开始下降,而在SBM(+ 3%葡萄糖)和SBS(+ 2%或3%葡萄糖)中,CP消失率的降低与时间无关。此外,葡萄糖处理显著降低了所有饲料原料的DM和CP的有效降解率(Pe),葡萄糖水平升高时,这种降低更为明显。添加3%葡萄糖时,CP的Pe分别降低了6%(FFSB)、16%(SBM)和18%(SBS)。葡萄糖补充(包括加水和热处理)所观察到的效果的解释是葡萄糖在这些条件下的还原活性。